VARIAN ASSOCIATES UNITY INOVA NMR SPECTROMETER CONSOLE

本应用程序的目的是用瓦里安联合UNITY INOVA升级核磁共振生理化学实验室的GE Omega核磁共振光谱仪。具体来说，升级将取代现有系统的所有元素（控制台、探头、室温垫片等），除了磁铁。然而，磁体将被翻新（断电，更换密封，重新通电，绘制磁场图，并进行超级填充）。布里格姆妇女医院医学部心脏病科生理化学核磁共振实验室目前支持十个NIH资助的项目。美国国立卫生研究院的这些项目是基于一些机构：布里格姆妇女医院、贝斯以色列女执事医疗中心和波士顿大学医学院。由生理化学核磁共振实验室支持的NIH项目主要研究分离的灌注小鼠和大鼠心脏。其他研究的生物系统包括完整的大鼠和小鼠腿，分离的细胞和提取样本。GE Omega核磁共振光谱仪已经使用了10年，升级的理由是为支持的项目提供重要的新功能，并为用户社区提供更可靠和可用的资源。当前NIH资助项目的科学目标要求当前系统无法满足的系统性能水平。这在离体灌注小鼠心脏的研究中尤其明显，其中31P核磁共振实验中可用的信噪比和时间分辨率不够。瓦里安UNITY INOVA控制台将大大提高信号噪声性能。瓦里安系统目前的噪声数据比通用欧米茄新出厂时的噪声数据至少低2db。此外，十年的艰苦使用已经降低了许多关键部件的性能，特别是探头。更换这些旧探头也会增加信噪比。第二个担忧是通用欧米茄的可靠性。GE Omega系统的许多关键部件不再可用。当发生故障时，零件必须送去修理或翻新。这导致越来越长时间无法使用该仪器进行所有研究。对仪器时间的巨大需求和许多项目研究（例如，处于特定发育阶段的转基因动物和肥大的外部实验模型）的时间关键性质使得随机和长时间的不可用非常昂贵和破坏性。瓦里安UNITY INOVA将大大提高可靠性和稳定、可预测的可用性。

The objective of this application is to upgrade the GE Omega NMR spectrometer of the NMR Laboratory for Physiological Chemistry with a Varian Associates UNITY INOVA. Specifically, the upgrade will replace all elements of the existing system (console, probes, room temperature shims, etc.) except for the magnet. The magnet will, however, be refurbished (de-energized, seals replaced, re-energized, field mapped, and supercon shimmed). The NMR Laboratory for Physiological Chemistry in the Cardiology Division of the Department of Medicine at Brigham and Women's Hospital currently supports ten NIH funded projects. These NIH projects are based at a number of institutions: the Brigham and Women's Hospital, the Beth Israel Deaconess Medical Center, and the Boston University School of Medicine. The NIH projects supported by the NMR Laboratory for Physiological Chemistry are primarily studying the isolated perfused mouse and rat heart. Other biological systems studied include intact rat and mouse leg, isolated cells, and extract samples. The rationale for upgrading the GE Omega NMR spectrometer, which is now ten years old, is to provide significant new capabilities to the supported projects and to provide a more reliable and available resource for the user community. The scientific aims of the current NIH funded projects demand a level of system performance that can not be met by the current system. This is especially evident in the studies of the isolated perfused mouse heart where the signal to noise and time resolution available in 31P NMR experiments is not adequate. The Varian UNITY INOVA console will have much improved signal to noise performance. Current noise figures for Varian system are at least 2 dB below those of GE Omega when it was new. In addition, ten years of hard use have degraded the performance of many critical components, in particular, the probes. Replacement of these old probes will also increase signal to noise. A second concern is the reliability of the GE Omega. Many critical components of the GE Omega system are no longer available. When breakdowns occur, parts must be sent out for repair or refurbishment. This is resulting in increasingly long periods of time when the instrument is unavailable for all studies. The great demand for instrument time and the time critical nature of many project studies (e.g., transgenic animals of a specific stage of development and exoerimenta1 models of hypertrophy) makes random and lengthy periods of unavailability very expensive and disruptive. The Varian UNITY INOVA will have vastly improved reliability and constant, predictable availability.