MODEL 377 DNA SEQUENCER AND ACCESSORIES

Dana-Farber癌症研究所建立了分子生物学核心设施，为研究所实验室提供方便和具有成本效益的各种分子生物学技术和试剂，如寡核苷酸，合成肽，微蛋白测序，质量分析，DNA测序，基因分型和蛋白质配体分析。 随着这些服务的建立和使用的增加，仪器不断升级，更换和增加新的仪器。 自动化DNA测序和基因分型最初成立于1990年，经历了所有服务中最引人注目的增长。 每年，成功测序反应的数量增加了35%，并且仍然没有显示出稳定的迹象。目前用于DNA测序和基因分型的仪器包括三个型号373 A测序仪和一个型号377测序仪，全部来自PE Biosystems，第一个型号373最初于1990年购买。 一台2400型、一台9600型和一台9700型热循环仪用于染料终止剂循环测序。该设施与DNA合成设施相结合，后者为DNA测序和基因分型项目提供寡核苷酸。 DNA合成设施目前使用一个型号3948和一个型号394，均来自PE Biosystems。该提案要求提供资金，用于从PE Biosystems购买一台377型自动DNA测序仪（容量升级为96台）和从MJ Research购买一台Tetrad热循环仪，以扩大容量，并通过更换一些较旧的373型测序仪来升级设施仪器。 通过购买这些仪器，该设施将能够改变到一个96格式的所有方面的DNA测序和基因分型协议，以更有效的运作。 研究所的承诺和一个行之有效的收费制度，以支付运营成本是到位的设施扩建。

Dana-Farber Cancer Institute established the Molecular Biology Core Facilities to provide Institute laboratories convenient and cost-effective access to various molecular biology techniques and reagents such as oligonucleotides, synthetic peptides, microprotein sequencing, mass analysis, DNA sequencing, genotyping, and protein-ligand analysis. As these services became established and usage increased, the instrumentation has been continually upgraded, replaced and augmented by new instrumentation. Automated DNA sequencing and genotyping was first established in 1990 and has undergone the most dramatic growth of all the services. Each year the number of successful sequencing reactions has increased by 35 percent and still shows no signs of leveling off. The current instrumentation for DNA sequencing and genotyping includes three model 373A sequencers and one model 377 sequencer all from PE Biosystems, the first model 373 originally purchased in 1990. One model 2400, one model 9600 and one model 9700 thermal cycler are used for dye terminator cycle sequencing. This facility is integrated with the DNA synthesis facility which provides oligonucleotides for DNA sequencing and genotyping projects. The DNA synthesis facility currently uses one model 3948 and one model 394, both from PE Biosystems. This proposal requests funds to purchase a model 377 automated DNA sequencer with 96 capacity upgrade from PE Biosystems and a Tetrad thermal cycler from MJ Research to expand the capacity as well as upgrade the facility instrumentation by replacing some of the older model 373 sequencers. By purchasing these instruments, the facility will be able to change over to a 96 format for all aspects of the DNA sequencing and genotyping protocols for a more efficient operation. Institute commitment and a proven chargeback system to cover operating costs are well in place for the expansion of the facility.