PHILIPS XL30 SCANNING ELECTRON MICROSCOPE

传统的生物材料扫描电子显微镜存在严重的局限性。这些问题包括分辨率的限制，缺乏反向散射检测，以及无法在低加速电压下对未涂覆的非导电样品进行成像。所有这些能力都是许多NIH资助的研究人员所迫切需要的，而亚利桑那大学缺乏这些能力严重限制了我们的生产力和进步。因此，我们正在申请资金购买一台飞利浦XL30扫描电镜，该扫描电镜配备了传统的二次电子探测器，以便在高真空条件下进行最佳检测，以及一台背散射电子探测器。飞利浦XL30 SEM被选为市场上的仪器，其功能使其成为最适合我们主要用户群体需求的仪器。这些需求包括：(a)在低电压下获得固定的、临界点干燥的、但未涂覆的样品表面的高分辨率二次电子图像，(c)获得未涂覆样品的背散射电子图像，以揭示内部结构以及表面结构标记的重金属（如胶体金）分布；(d)以摄影视频或数字格式记录这些图像，以供随后的演示、存档和/或图像分析。研究计划表明，我们迫切需要一台扫描电镜，不仅要取代医学成像学院现已停产、已有22年历史的传统扫描电镜，还要为8个nih资助的主要用户提供高分辨率的传统扫描电镜功能。以及亚利桑那大学各院系中至少六名由美国国立卫生研究院资助的未成年用户，以及南加州大学的一名研究员，该研究员与她的项目所需的首席研究员合作使用电子显微镜。

There are severe limitations for conventional scanning electron microscopy of biological material. These include limitations on resolution, lack of back-scattered detection, and the inability to image uncoated, non-conductive samples at low accelerating voltages. All of these capabilities are critically needed by a number of NIH funded investigators and lack of access to them at the University of Arizona is seriously limiting our productivity and progress. As a result, we are requesting funds to purchase a Philips XL30 SEM equipped with a conventional secondary electron detector for optimal detection under high vacuum conditions, and a backscattered electron detector. The Philips XL30 SEM was selected as the instrument on the market having the features which make it the most suitable instrument for the needs of our major user group. These needs include the ability to: (a) obtain high resolution secondary electron images at low voltage on the surfaces of fixed, critical-point dried, but uncoated sample, (c) obtain backscattered electron images of uncoated samples to reveal internal structure as well as the distribution heavy metal (e.g., colloidal gold) labeling of surface structures; (d) record these images in photographic video, or digital format for subsequent presentation, archiving, and/or image analysis. The research plans demonstrate our immediate and critical need for an SEM not only to replace our now defunct, 22 year-old conventional SEM at the College of Medicine Imaging Faculty, but also to provide high-resolution conventional SEM capabilities to eight NIH-funded major users, and at least six NIH-funded minor users in a variety of departments within the University of Arizona and one investigator from the University of Southern California who collaborates with the Principal Investigator for the electron microscopy required for her project.