PROCISE 491 CLC PROTEIN SEQUENCER

这是一个NIH-NCRR共享仪器补助金，用于为印第安纳州大学医学院购买ABI Procise 491 cLC蛋白测序仪。 我们有四个由NIH资助的核心用户组，他们将占据90%的可用仪器时间。这些项目的名称是：1）确定蛋白激酶磷酸化靶标的基因组方法，2）鉴定负责调节支链α-酮酸脱氢酶和丙酮酸脱氢酶复合物的磷酸蛋白磷酸酶，3）鉴定和结构表征钙摄取和释放所需的心肌肌浆蛋白，4）肝星状细胞维甲酸代谢酶的鉴定。还有另外10个核心的NIH资助的用户表示对蛋白质测序感兴趣，并将占用仪器剩余的10%时间。 在医学院校园里没有普遍可用的蛋白质测序仪。Procise cLC将被放置在印第安纳州大学医学院新成立的跨学科结构生物学中心，其目标是为有兴趣表征生物大分子结构特性的研究人员提供最先进的仪器和教师顾问。 该中心的日常运作将由一名全职科学主任协调。 印第安纳州大学和生物化学与分子生物学系已承诺提供资金，用于支持科学主任和维护Procise cLC蛋白测序仪。

This is an NIH-NCRR Shared Instrumentation Grant to purchase an ABI Procise 491 cLC protein sequencer for the Indiana University School of Medicine. We have a core of four NIH-funded user groups who will occupy 90 percent of the available instrument time. The titles of the projects are: 1) Genomic Approach to Determining Phosphorylation Targets for Protein Kinases, 2) Identification of the Phosphoprotein Phosphatases Responsible for Regulation of the Branched-Chain alpha-Ketoacid Dehydrogenase and Pyruvate Dehydrogenase Complexes, 3) Identification and Structural Characterization of Cardiac Sarcoplasmic Proteins Required for Calcium Uptake and Release, 4) Identification of retinoid metabolizing enzymes in hepatic stellate cells. There is an additional core of ten other NIH-funded users who have expressed an interest in protein sequencing and will occupy the remaining 10 percent of the instrument time. There is no generally available protein sequencer on the Medical School campus. The Procise cLC will be placed in the newly formed interdisciplinary Center for Structural Biology at Indiana University School of Medicine whose goal is to provide state-of-the-art instrumentation and faculty consultants for researchers interesting in characterizing the structural properties of biologic macromolecules. The day-to-day operations of the Center will be coordinated by a full-time Scientific Director. Indiana University and the Department of Biochemistry and Molecular Biology have committed funds for the support of the Scientific Director and for maintenance of the Procise cLC protein sequencer.