PEPTIDE PRINTER FOR HIGH-THROUGHPUT SCREENING

描述（申请人摘要）：我们将建立一个肽“打印机”的能力 通过原位合成，产生包含至少1000个独特的 在单个基底（载玻片或膜）上的肽。合成将是 通过使用喷墨技术连续微分配氨基酸来执行。 我们的10通道打印头，具有每秒400个点的有效速率， 通道，将允许在1分钟内打印4，000个氨基酸点。 使用微规模合成，洗涤、封闭、脱保护和偶联循环将 从目前宏观规模合成的一小时缩短到不到一半 一个小时，从而产生每小时多于一个残留物的合成速率。 15聚体肽将在不到24小时内合成。增加的 密度和减少的合成时间将导致更便宜、更高的 密度阵列比目前可用的。第一阶段表明， 微分配可用于合成高纯度肽。在第二阶段，我们 将建立一个原型打印机能够合成阵列， 1000种不同的肽该原型系统将用于合成 基于良好表征的蛋白质的肽阵列。这些肽阵列将 用于验证大规模阵列的综合。肽阵列将 在普罗克特& Gamble的药物发现研究中进行生产和评估。 拟定商业应用： 肽库用于药物筛选/发现和免疫学研究 （用于诊断和蛋白质表位作图的抗体）。 肽文库阵列还 用于定义包括所有受体-配体的蛋白质-蛋白质相互作用结构域的有效工具， 抗体-抗原相互作用 将使用具有可定制肽的肽库芯片 所有的一切。

DESCRIPTION (applicant's abstract): We will build a peptide "printer" capable of producing, by in situ synthesis, arrays containing at least 1000 unique peptides on a single substrate (glass slide or membrane). The synthesis will be performed by successively microdispensing amino acids using ink-jet technology. Our ten channel printheads, having effective rates of 400 spots per second per channel, will allow 4,000 amino acid spots to be printed in under 1 minute. Using micro-scale synthesis, wash, block, deprotect, and coupling cycles will be reduced from one hour for current macro-scale synthesis, to less than half an hour, thus yielding a synthesis rate of more than one residue per hour. Fifteen-mer peptides will be synthesized in less than 24 hours. The increased density and decreased synthesis time will result in less expensive, higher density arrays than are currently available. Phase I demonstrated that the micro-dispensing can be used to synthesize high purity peptides. In Phase II we will build a prototype printer capable of synthesizing arrays with at least 1000 different peptides. The prototype system will be used to synthesize peptide arrays based on well-characterized proteins. These peptide arrays will be used to validate the synthesis for a large-scale array. Peptide arrays will be produced and evaluated in Proctor & Gamble's drug discovery research. PROPOSED COMMERCIAL APPLICATION: Peptide libraries are used for drug screening/discovery and immunological research (antibodies for diagnostics and protein epitope mapping). Peptide libraries arrays are also an efficient tool for defining protein-protein interaction domains including all receptor-ligand, antibody-antigen interaction. A peptide library-chip with customizable peptide will be used by all of the above.