New and Versatile Approach to Synthesis of chemRNA

mRNA的表达通常需要苛刻的程序或特定的酶促步骤。我们提出了一种利用化学连接RNA （chemRNA）的替代策略，旨在增强体外转录等程序，提高RNA稳定性并允许位点特异性修饰。chemRNA将通过酰胺键和三唑铜键反应进行连接。这种模块化方法具有独特的优势，允许从天然和修饰的序列中生产蛋白质。我们的目的是优化化学rna的合成，研究化学rna在生物机制中的保真度，并从增强的化学rna合成蛋白质。我们认为这项工作属于BBRSC的三个优先领域。chemRNA在工业生物技术中具有很好的规模化应用潜力。它是一种新的合成方法，是合成生物学进步的关键，可以作为生物科学进步的通用技术。AfS, ENWW

mRNA expression often requires demanding procedures or specific enzymatic steps. We propose an alternative strategy utilising chemically ligated RNA (chemRNA) with the aim to enhance procedures such as in vitro transcription, improve RNA stability and allow for site specific modifications. chemRNA will be ligated, using amide linkages and triazole copper click reactions. This modular approach has a unique advantage allowing for protein production from both natural and modified sequences. Our intention is to optimise the synthesis of chemRNA, investigate the fidelity of chemRNA in biological mechanisms and synthesise proteins from enhanced chemRNA. We consider this work to fall within three priority areas of the BBRSC. chemRNA has good potential for scale-up applications in industrial biotechnology. It is a new synthetic approach that is key to advances in synthetic biology and can be used as a universal technique for the advancement of the biosciences. AfS, ENWW