CORE--SYNTHETIC ANTIGEN LABORATORY

合成抗原设施提供高度人工合成的多肽 向德克萨斯大学M.D.安德森大学的教职员工致敬 癌症中心。该设施位于B8.4806(544英尺/2)房间， 配备了一个特殊的增强型排气系统来处理HF。 设施工作人员为研究人员提供咨询 关于合成肽。该机构正在提供新肽 合成器和高效液相色谱(HPLC)设备 这将使该设施目前的产能翻一番，并降低其 周转时间到了。该设施的按存储容量使用计费账户的资金涵盖 材料成本和一名研究调查员的工资。一个 监督委员会由John McMurray，P.H.D.，Benoit组成 DeCrombrogghe，M.D.和Ralph Arlinghaus，P.H.D.的主要功能 这个委员会将审查该设施的运行情况，使 提出改进建议，确定优先顺序，确保满意度 用户的数量。去年生产的122个多肽中有93个(7/1/96至 6/30/97)是为同行资助的调查人员编写的，反映了 工厂的目标是向最具竞争力的公司提供高质量的试剂 和富有成效的教职员工。这些多肽被提供给总共 21个用户，其中14个是由同业资助的。自从上一次竞标更新以来， 合成了936个多肽，其中612个是给同行资助的用户的 (1991年7月1日至1997年6月30日)。一般而言，提供给 用户通常是90%或更高，一些多肽是以一种 纯度大于95%。以下是提供的多肽类型的一个示例 过去的一年是为拉里·埃特金博士合成的42个氨基酸的多肽 (分子遗传学系)。该多肽的序列为 来源于埃特金博士发现的一种独特的锌指结构域。研究 伦敦的一个物理化学小组对这种多肽进行了研究， 英格兰，建立了这一领域的结构。关键因素是 多肽供应量大，成本比成本低 外部竞争对手。另一种检查是磷酸酪氨酸肽。 提供给戈登·米尔斯博士(分子肿瘤学系)。这 合成需要用另一种方法进行磷酸化。 这一领域的许多人。通常，将磷酸盐添加到Y残基中 合成后，由于氧化敏感物质的存在 残基(例如，两个蛋氨酸)，一种受保护的磷酸化氨基酸 合成阶段使用了衍生物(tBOC 二甲基磷酸酪氨酸)。磷酸酪氨酸肽的得率为98% 纯洁。

The Synthetic Antigen Facility provides synthetic peptides in a highly purified state to faculty members of the University of Texas M.D. Anderson Cancer Center. This facility is located in room B8.4806 (544 feet/2) and is equipped with a special enhanced exhaust system for handling HF. Facility staff members provide consultation to the research staff regarding synthetic peptides. The institution is provide new peptide synthesizers and high-performance liquid chromatography (HPLC) equipment that will double the facility's current capacity and decrease its turnaround time. Funds from the facility's charge-back account cover the cost of materials and the salary for one research investigator. An oversight committee exists consisting of John McMurray, P.h.D., Benoit deCrombrugghe, M.D., and Ralph Arlinghaus, P.h.D. The major functions of this committee are to review the operation of the facility, make suggestions for improvement, set priorities, and ensure the satisfaction of the users. Ninety-three of the 122 peptides made last year (7/1/96 to 6/30/97) were produced for peer-funded investigators, reflecting the facility's goal to provide high-quality reagents to the most competitive and productive faculty members. These peptides were provided to a total of 21 users, 14 of whom were peer funded. Since the last competitive renewal, 936 peptides were synthesized, 612 of which were for peer-funded users (7/1/91 to 6/30/97). In general the purity of the peptides provided to users was routinely 90% or higher,a nd some peptides were supplied at a purity greater that 95%. One example of the type of peptides supplied this past year is a 42-amino-acid peptide synthesized for Dr. Larry Etkin (Department of Molecular Genetics). The sequence of this peptide was derived from a unique zinc-finger domain discovered by Dr. Etkin. Research studies involving this peptide by a physical chemistry group in London, England, established the structure of this domain. The key factors were the large amount of peptide supplied and the low cost compared with costs of outside competitors. Another examine is a phosphotyrosine peptide supplied to Dr. Gordon Mills (Department of Molecular Oncology). This synthesis required an alternative method of phosphorylation from that used by many in the field. Normally, the phosphate is added to the Y residue after synthesis, but because of the presence of oxidation-sensitive residues (e.g., two methionines), a protected, phosphorylated amino-acid derivative was used for the synthesis phase (tBOC dimethylphosphotyrosine). The phosphotyrosine peptide was provided at 98% purity.