CORE--MOLECULAR CYTOGENETICS FACILITY

描述（由申请人提供）：分子细胞遗传学核心设施 为癌症中心的研究人员提供细胞遗传学和分子DNA分析， 新鲜的细胞和组织，建立的和体细胞系，人肿瘤和 小鼠肿瘤，特别是来自转基因小鼠的肿瘤。此核心是新的，因为 这是癌症中心补助金的最后一次提交， 计划1、3、5和6成员的要求。细胞遗传学服务包括 成纤维细胞、淋巴母细胞或体细胞常规Giemsa（G）显带和C显带 细胞杂交系用于确定核型的目的。该服务还 包括利用荧光原位杂交（FISH）， 这些核型的澄清，包括m-FISH（FISH）和 SKY（光谱核型分析），使用24种不同的颜色来分析 染色体变化FISH也被用来研究染色体的变化， 肿瘤中的间期细胞（尤其是石蜡切片），骨髓中 移植和基因的染色体定位。分子服务 该核心的组成部分包括肿瘤的微卫星分析， 肿瘤的杂合性、突变分析和骨的嵌合体研究 骨髓移植在某些情况下，为了提供服务，组织培养 服务是必要的，包括：一般的增长，维护和冻结 病人？细胞系;淋巴细胞转化产生永久性 从细胞系和外周血制备DNA; 并根据需要产生体细胞系。在其他情况下， 执行服务，新的，专门的服务，包括比较基因组 杂交（CGH）和嵌合体分析。这些 增加了两个计算机辅助分析系统， 显微镜和DNA测序仪为响应用户请求， 该研究所积极开发分子细胞遗传学新技术。

DESCRIPTION (provided by applicant): The Molecular Cytogenetics Core Facility provides Cancer Center investigators cytogenetic and molecular DNA analysis of fresh cells and tissue, established and somatic cell lines, human tumors and mouse tumors, particularly those from transgenic mice. This Core is new since the last submission of this Cancer Center Grant and was developed at the request of members of Programs 1, 3, 5 and 6. Cytogenetic services consist of routine Giemsa (G)-banding and C-banding on fibroblast, lymphoblast or somatic cell hybrid lines for the purpose of determining karyotypes. The service also includes utilization of fluorescence in situ hybridization (FISH) for better clarification of these karyotypes, including both m-FISH (multicolor FISH) and SKY (spectral karyotyping), that uses 24 different colors to analyze chromosomal changes. FISH is also utilized to study chromosomal changes in interphase cells in tumors (especially in paraffin sections), in bone marrow transplants, and for chromosomal localization of genes. The molecular service of this Core consists of microsatellite analysis of tumors for loss of heterozygosity, mutation analysis of tumors, and chimerism studies of bone marrow transplants. In some instances to perform the services, tissue culture services are needed and include: general growth, maintenance, and freezing of patients? cell lines; transformation of lymphocytes to generate permanent lymphoblast lines; preparation of DNA from cell lines and peripheral blood; and generation of somatic cell lines as needed. In other instances, to perform the services, new, specialized services including comparative genomic hybridization (CGH) and chimerism analysis have been developed. These analyses have been facilitated by the addition of two computer-assisted microscopy units and a DNA sequencer. In response to user requests, the facility actively develops new technology in molecular cytogenetics.