FOODBORNE NORWALK-LIKE VIRUSES: ARE THEY INFECTIOUS?

说明(改编自应用程序) 本申请是根据RFA公告DK-00-005提交的， 食源性疾病、胃肠道和肾脏并发症。一项新计划 为确定诺沃克病毒的传染性和 还提出了其他类似诺瓦克的病毒。类似诺沃克的病毒是单一的， 食源性疾病的最重要原因，但迄今为止还没有系统 确定这些病毒的传染性是存在的。两种方法将是 使用诺沃克病毒株来确定这些病毒的传染性 病毒：(1)细胞内病毒体外培养方法的建立 培养和(2)开发生物传感方法以允许快速 检测可能具有传染性的诺瓦克样病毒。以下几个特点构成了 诺沃克病毒，NLV的原型毒株，一个有吸引力的候选病毒 诺瓦克样病毒体外感染性检测方法的研究进展(一) 已确定在人类中具有传染性的病毒库存将是 可用；(Ii)检测完全或部分诺瓦克的敏感方法 已经发展了病毒复制，包括RT-PCR分析， 免疫荧光检测和原位RT-PCR检测；(Iii)新鲜粪便样本 含有诺沃克病毒(来自人类实验感染研究)的有 预计在赠款期间可供使用；(Iv)和高度免疫的动物 急性期和恢复期的人血清可用于中和 化验。最初使用诺沃克病毒开发的检测方法将被 推广到其他类似诺瓦克的病毒。从我们的研究中获得的结果 研究将产生关于复制类诺瓦克的新知识 病毒和开发用于评估确定免疫相关因素的系统 从感染和测量暴露在不同环境中的病毒活性 环境条件。

DESCRIPTION (adapted from the application) This application is submitted in response to RFA announcement DK-00-005, "Foodborne Illnesses, Gastrointestinal and Renal Complications." A new program for developing a strategy for determining the infectivity of Norwalk virus and other Norwalk-like viruses is proposed. Norwalk-like viruses are the single, most important cause of foodborne illness, but to date no system for determining the infectivity of these viruses exists. Two approaches will be pursued using the Norwalk virus strain to determine the infectivity of these viruses: (1) development of an in vitro method for virus cultivation in cell culture and (2) development of a biosensoring method to permit the rapid detection of potentially infectious Norwalk-like viruses. Several features make Norwalk virus, the prototype strain of NLVs, an attractive candidate to use in the development of in vitro measures of Norwalk-like virus infectivity: (i) virus stock for which infectivity in humans has been determined will be available; (ii) sensitive methods for detecting complete or partial Norwalk virus replication have been developed, including RT-PCR assay, immunofluorescence assays, and in situ-RT-PCR assays; (iii) fresh stool samples containing Norwalk virus (from human experimental infection studies) are expected to be available during the grant period; (iv) and hyperimmune animal and acute and convalescent human sera are available for use in neutralization assays. The assays developed initially using Norwalk virus will then be generalized to other Norwalk-like viruses. The results obtained from our studies will generate new knowledge about the replication of Norwalk-like viruses and develop systems for assessing determining correlates of immunity from infection and measuring virus viability after exposure to different environmental conditions.