Effect of Exercise/Amino Acids on Protein Metabolism

描述(申请人？S摘要)：拟研究内容涉及 急性耐力运动和混合氨基酸对人体活体的影响 补充剂对骨骼肌蛋白质合成的影响及其调节 细胞内信号转导。在具体目标1中，健康的成年人将 学习。蛋白质合成将通过肌肉蛋白质的富集量进行评估 稳定同位素亮氨酸示踪剂。还将对以下活动进行评估 P70S6K和PHAS-1(eLF4E-BP1)，这两个关键信号蛋白参与了 翻译启蒙。在特定目标II中，运动和氨基酸的影响 肾功能衰竭受试者体内的酸补充将被研究。 方法包括L-1-13C亮氨酸输注和肌肉活检。 之后。活检组织中PHAS-1和p70S的磷酸化速度将是 用凝胶电泳法和蛋白质印迹法检测。部分合成比率 将通过蛋白质和蛋白质的质谱学测量来计算 L-1-13C亮氨酸的胞浆浓缩。团体将接受以下两种治疗之一 生理盐水输注、运动或运动加氨基酸输注。研究对象将 在自行车功率计上锻炼一小时。有很多问题没有得到回答 关于身体如何调节两者中蛋白质代谢的问题 健康和疾病状态，如肾衰竭。这些研究的目标是 为针对特定目标的潜在治疗提供基础 机械装置。

DESCRIPTION (Applicant?s abstract): The research proposed involves studying in humans in vivo the effect of acute resistance exercise and mixed amino acid supplementation on skeletal muscle protein synthesis and the regulation of intracellular signal transduction. In Specific Aim 1, healthy adults will be studied. Protein synthesis will be assessed by muscle protein enrichment of stable isotope leucine tracer. There will also be assessment of the activity of p70S6k and PHAS-1 (eLF4E-BP1), two key signaling proteins involved in translation initiation. In Specific Aim II, the effects of exercise and amino acid supplementation in vivo in subjects with a renal failure will be studied. The methods include infusion of L-1-13C leucine and a muscle biopsy before and after. The phosphorylation rate of PHAS-1 and p70S from biopsy tissue will be measured by gel electrophoresis and Western blot. A fractional synthetic rate will be calculated from measurements by mass spectrometry of protein and cytosolic enrichment of L-1-13C leucine. Groups will be treated either with saline infusion, exercise, or exercise and amino acid infusion. Subjects will be exercised for one hour on a cycle ergometer. There are many unanswered questions about how the body regulates the metabolism of protein in both healthy and disease states, such as renal failure. The goal of these studies is to provide a foundation leading to potential treatments targeted at particular mechanisms.