GROWTH FACTORS AND LIGHT HISTORY IN PHOTORECEPTOR RESCUE

广泛的长期目标是了解视网膜如何适应 自身对环境光的变化，以保护感光细胞 并阐明这种适应的机制， 系统被触发，最终，操纵这个系统， 增加感光细胞对变性的抵抗力。 重点 这项研究计划的一部分是描述基因和蛋白质 表达作为视网膜适应的函数， 循环光，并了解这种适应如何保护 光感受器的退化 为了实现这一目标， bFGF、aFGF、FGF受体-1、CNTF、CNTF受体- α、BDNF、trk B、IGF-1和IGF-1受体，以及原癌基因 bcl-2将通过进行北方和 Western印迹分析。 随后，原位杂交和 将进行免疫细胞化学以定位表达。 在 此外，成年大鼠视网膜中的这种适应系统由明亮的 循环光，以保护光感受器从退化也将是 考察

The broad, long-term objective is to understand how retina can adapt itself to environmental light changes to protect photoreceptor cells from degeneration and to elucidate the mechanism whereby this adaptation system is triggered, and eventually, to manipulate this system to increase photoreceptor cell resistance to the degeneration. The focus of this research proposal is to characterize the genes and protein expression as a function of retinal adaptation in response to bright cyclic light, and to understand how this adaptation protects photoreceptors from degeneration. To achieve this goal, the gene and protein expressions of bFGF, aFGF, FGF receptor-1, CNTF, CNTF receptor- alpha, BDNF, trk B, IGF-1 and IGF-1 receptor, as well as proto-oncogene bcl-2 will be quantitatively characterized by performing Northern and Western blot analysis. Afterward, in situ hybridization and immunocytochemistry will be performed to localize the expression. In addition, such adaptation system in adult rat retina triggered by bright cyclic light to protect photoreceptors from degeneration will also be examined.