DISSECTION OF AN RNA CONFORMATIONAL CHANGE

描述 RNA参与催化翻译的基本过程， mRNA剪接。催化这些过程的复合物（核糖体 和剪接体）也含有蛋白质， 主要或完全通过稳定活性结构和促进 RNA组分的构象变化。要了解的手段， RNA可以加速多步反应，蛋白质可以促进 通过RNA的催化，重要的是要了解其内在的能力， 以及RNA在进行构象重排时的局限性。RNA 单独加速组I的两步剪接反应， 构象重排RNA单独加速两步剪接 进行构象重排的第一组。RNA单独加速 四膜虫Ⅰ类内含子两步剪接反应 嗜热菌，使其成为详细研究的理想模型系统。一 构象变化发生在两个化学步骤之间， 用于切割的3’-剪接位点。相互作用被打破和形成 在这一重排过程中，将使用动力学 方法和化学探测，以及单个碱基的动力学， 当3-剪接位点进入活性位点时发生的配对变化 将使用荧光方法测定。希望A 对RNA中单个构象变化的详细了解将有助于 我们对RNA动力学的理解。

DESCRIPTION RNA is involved in catalysis of the essential processes of translation and mRNA splicing. The complexes that catalyze these processes (the ribosome and spliceosome) also contain protein, which is proposed to function largely or entirely by stabilizing the active structures and facilitating conformational changes of the RNA components. To understand the means by which RNA can accelerate multi-step reactions and protein might facilitate catalysis by RNA, it is important to understand the intrinsic capabilities and limitations of RNA at performing conformational rearrangements. RNA alone accelerates the two-step splicing reaction of a group I performing conformation rearrangements. RNA alone accelerates the two-step splicing of a group I performing conformation rearrangements. RNA alone accelerates the two-step splicing reaction of a group I intron from Tetrahymena thermophila, rendering this an ideal model system for detailed study. A conformational change occurs between the two chemical steps that positions the 3'-splice site for cleavage. Interactions that are broken and formed during the course of this rearrangement will be identified using kinetic approaches and chemical probing, and the kinetics of individual base- pairing changes that occur as the 3-splice site enters the active site will be determined using a fluorescence approach. It is hoped that a detailed understanding of a single conformation change in RNA will advance our understanding of RNA dynamics more generally.