CORE--CELLULAR AND MOLECULAR BIOLOGY

这一核心将促进使用最先进的蜂窝和 分子生物学技术在该计划的所有项目中 项目核心的职责将包括 两个分离的相关功能：1）组织培养单元将 提供组织培养细胞和必要的专业知识来完成 细胞生物学协议在个人的建议和2） 分子生物学单位将定量从收获的特定mRNA 组织和培养细胞，如五个项目中的每一个所概述的。在 上一个程序项目，分子生物学单位是一个部分， 分子遗传学核心分子技术的广泛应用， 所有研究者以及组织培养专业知识的需求， 其中几个项目导致了当前核心的发展， 在这个应用程序中概述。方案、设备、试剂和 核心中还提供了许多其他技术的专业知识。 这些包括原位Northern、Southern和Western印迹分析， 杂交组织化学，免疫细胞化学，基因亚克隆 用于核糖探针开发和核糖探针合成的片段。如果 如果需要，这些程序将在研究期间提供给研究者。 建议的研究过程。

This core will facilitate the use of state-of-the-art cellular and molecular biology technologies in all of the projects in the Program Project. The responsibilities of the Core will involve the performance of two separate by related functions: 1) The Tissue Culture unit will provide tissue culture cells and the expertise necessary to complete the cellular biology protocols in the individual proposals and 2) The Molecular Biology unit will quantify specific mRNAs from harvested tissue and cultured cells as outlined in each of the five projects. In the previous program project, the molecular biology unit was a part of the Molecular Genetics Core. The expanded use of molecular techniques by all investigators as well as the need for tissue culture expertise in several of the projects resulted in the development of the current Core outline din this application. The protocols, equipment, reagents and expertise for a number of other techniques are available in the Core. These include Norther, Southern and Western blot analysis, in situ hybridization histochemistry, immunocytochemistry, subcloning of gene fragments for riboprobe development, and riboprobe synthesis. If required, these procedures will be provided to the investigators during the course of the proposed studies.