Making vitamin H sustainable - developing biotin overproduction approaches

我的研究目标是创造一种功能强大、经过改进的Biob酶，以全面提高生物素的产量。我的目标是创造出比现有的大肠杆菌Biob具有更快周转速度的Biob酶的变体，并使我们能够克服生物素生物合成途径中的第一个动力学瓶颈。我希望实现这一点的方法是通过合理的设计在大肠杆菌中创造这种酶的突变。这将使我能够确认在天然酶中可以获得的影响范围以及这些残留物对反应活性的作用。

My research aims to create a functional and improved BioB enzyme to overall improve the production of biotin. My objectives are to create variants of BioB enzyme that have a faster turnover rate than existing E. coli BioB and allow us to overcome the first kinetic bottleneck in the biotin biosynthesis pathway. The ways in which I hope to achieve this is through rational design create mutants of this enzyme in E. coli. This will enable me confirm the range of impact that may be accessible in the natural enzymes and the role of these residues on the reactivity.