LOW RISK ILLICIT DRUG LEVEL MONITORING

描述（由申请人提供）：拟议研究的目标是开发两个与自动分析阅读器接口的原型药物分析仪，通过表面增强拉曼免疫测定（SERIA）对非法药物水平进行低风险监测。第一个原型将被放置在临床环境中。本研究将包括从青少年评估和治疗中心的来访者收集的尿液和唾液样本。我们将通过标准尿液分析方法与传统免疫测定方法进行比较，交叉验证这项工作。这将为我们提供一个可信的验证和FDA批准的开始。第二个原型药物分析仪将被开发为一种多格式仪器，并优化以评估工程和自动化分析方法。该仪器将被设计为适应用于筛选药物样品的微孔板格式，用于单个药物分析的微样品瓶格式和简单的一步试纸格式。在这个项目的过程中，我们将进一步发展我们的检测技术，改进我们的药物模拟报告器的设计，优化我们检测甲基苯丙胺、安非他明和吗啡的方法，将我们的研究扩展到四氢大麻酚和可卡因，并开发相应的SERIA方法来检测这些药物。

DESCRIPTION (provided by applicant): The goal of the proposed research is to develop two prototype drug analyzers interfaced with automated analysis readers for low risk monitoring of illicit drug levels by Surface Enhanced Raman Immuno Assay (SERIA). The first prototype will be placed in a clinical setting. This study will include urine and saliva samples collected from clients of a juvenile assessment and treatment center. We will cross validate this work with a comparison to conventional immunoassay measurements through standard urinalysis methodology. This will provide us with a credible validation and a beginning for FDA approval.The second prototype drug analyzer will be developed as a multiformat instrument and optimized to evaluate the engineering and automated assay methodology. This instrument will be designed to accommodate a microwell plate format for screening drug samples, a microsample vial format for individual drug assays, and a simple one-step test-strip format.In the course of this project we will further the development of our assay technique by improving upon the design of our drug-analog reporters and optimizing our methodology for the detection of methamphetamine, amphetamine, and morphine by extending our study to include THC and cocaine, and developing corresponding SERIA methods for these drugs.