Crystallization Tools for Structural Genomics

描述：申请人？S摘要)一种集成的、自动化的蛋白质 结晶系统RoboHTC(机器人高通量结晶)将 由适用于以下方面的软件和硬件工具组成 大型蛋白质结晶学项目，如结构基因组学。 这些工具也将高度模块化，允许单独的家庭实验室 根据需要使用系统的一个或多个组件。这些工具 将解决当前结晶中的问题，这是 目标分离纯化后的蛋白质结晶学 蛋白。我们还将把这些工具与基于Web的Java小程序集成在一起 使研究人员可以定制结晶解决方案。这将是 使研究人员不必制作自己的结晶屏， 这是目前蛋白质结晶中最繁琐的任务之一。 整个结晶学社区都将从拟议的 研究。作为我们结晶数据库进一步发展的一部分 软件，我们将实现功能，使研究人员可以轻松地将 结晶数据进出蛋白质数据库。这些公开的 可访问的结晶数据然后可以指导其他研究人员在他们的 结晶的努力。随着蛋白质结晶学变得越来越 生物研究的重要方面，拟议的工具将使蛋白质 晶体结构测定是任何生物学研究中比较常规的一部分 程序。 建议的商业应用： 不可用

DESCCRIPTION: Applicant?s abstract) An integrated, automated protein crystallization system,RoboHTC (Robotic High-Throughput Crystallization), will be developed, consisting of software and hardware tools suitable for large-scale protein crystallography projects, such as structural genomics. These tools will also be highly modular, allowing individual home laboratories to use one or more components of the system as their needs demand. These tools will address current problems in crystallization, the rate-limiting step in protein crystallography, following isolation and purification of the target protein. We will also be integrating these tools with a Web-based Java applet to allow researchers to order custom crystallization solutions. This will alleviate researchers from having to make their own crystallization screens, which is presently one of the most tedious tasks in protein crystallization. The entire crystallographic community stands to benefit from the proposed research. As part of the further development of our crystallization database software, we will implement features to allow researchers to easily transfer crystallization data to and from the Protein Data Bank. These publicly accessible crystallization data can then guide other researchers in their crystallization efforts. As protein crystallography becomes an increasingly important aspect of biological research, the proposed tools will make protein crystal structure determination a more routine part of any biological research program. PROPOSED COMMERCIAL APPLICATION: Not Available