Rapid, Automated, Multi-Sample Purification of RNA

描述(由申请人提供)：在第二阶段，我们将开发一个完整的 全自动、高通量、低成本、多样本的mRNA提纯仪 和一次性样本盒。与目前可用的任何其他进程不同 对于信使核糖核酸的纯化，本仪器使用了一种电泳法的衍生品 我们公司以前为DNA开发的分离技术 结合一种新的信使核糖核酸分离和收集方法的纯化 进程。分离技术是强大的，因为它可以通过 多通道一次性样品盒与廉价样品盒的组合 加工仪器。该仪器及其供应品的供应情况 将节省大量在实验室进行医学和生物研究的时间 目前正在进行手动的mRNA分离。在第一阶段，我们测试了这个自动化的 方法，成功地纯化了多个样品中的mRNA。 不到30分钟。我们证明了这种提纯方法可以用于一种 起始细胞数量或组织重量的范围很大，但产量仍然很高 纯的、生物活性的信使核糖核酸。 我们将在第二阶段研发的仪器将提纯mRNA 直接从细胞或组织裂解液通过全自动，两个步骤 可并行处理多达96个样本的程序。这台仪器是 预计最终用户的成本不到10,000美元，可支配成本为 每件1.5美元。这些功能将使该乐器至少两次 比目前任何自动化产品的每份样品的成本快一半 可用。这种仪器将比竞争对手便宜好几倍。 产品，因此较小的实验室负担得起。 建议的商业应用： 这项工作中开发的方法和产品将由麦康奈尔商业化 以可销售的仪器和一次性盒式磁带的形式出现的研究公司 给全球50,000个分子生物学实验室。这些产品将极大地 提高研究人员研究遗传疾病、病毒感染和 分子水平上的细胞机制。

DESCRIPTION (provided by applicant): In Phase II, we will develop a fully automatic, high-throughput, low-cost, multi-sample mRNA purification instrument and disposable sample cassettes. Unlike any other process currently available for mRNA purification, this instrument uses a derivative of the electrophoretic separation technology that was previously developed by our company for DNA purification in conjunction with a novel mRNA separation method and collection process. The separation technique is powerful in that can be performed with the combination of a multi-channel, disposable sample cassette and an inexpensive processing instrument. The availability of this instrument and its supplies will save countless hours of medical and biological research in labs that currently perform manual mRNA isolation. In Phase I, we tested this automated method and showed that it satisfactorily purified multiple samples of mRNA in less than 30 minutes. We showed that the purification method could be used on a wide range of starting cell numbers or tissue weights and still yield highly pure, biologically active mRNA. The instrument that we will research and develop in Phase II will purify mRNA directly from the cell or tissue lysate by a fully automated, two step procedure that can process up to 96 samples in parallel. The instrument is projected to cost less than $10,000 to the end user, with disposable costs of $1.5 per sample. These features will make the instrument at least two times faster and half the cost per sample of any automated product currently available. The instrument will be several times less expensive than competitor products, and thus affordable to smaller labs. PROPOSED COMMERCIAL APPLICATION: The methods and products developed in this work will be commericalized by MacConnell Research Corp. in the form of instruments and disposable cassettes that can be marketed to the 50,000 molecular biology laboratories worldwide. These products will greatly enhance the capability of researchers studying genetic disease, viral infection and cellular mechanisms at the molecular level.