CORE--CYTOMETRY FACILITY

在空间、时间和功能水平上分析单个细胞和细胞群是现代免疫学、细胞生物学和发育生物学的核心。细胞术核心专注于提供光学显微镜或荧光活化细胞分选（FACS）技术，这些技术可以与特定的分子探针（如抗体、DNA、mhc四聚体、细胞内报告分子）结合应用，以解决与1型和2型糖尿病的发病机制、治疗和并发症相关的基本问题。核心将位于芭芭拉戴维斯中心，由2名FTE技术人员在D. Bellgrau博士的领导下操作。它将使用最近从美国国立卫生研究院和慈善基金获得的大型设备。将提供12项服务，包括标准的FACS分析和实体组织的免疫组织化学，以及更复杂的活细胞信号转导和亚细胞事件分析。将免费提供两周一次或每月一次的正式培训，并将为所提供的每项服务提供专家咨询。

Analysis of individual cells and cell populations at the spatial, temporal and functional level lies at the heart of modern immunology, cell biology and developmental biology. The Cytometry Core focuses on the provision of light microscopal or fluorescence activated cell sorting (FACS) technologies which can be applied in combination with specific molecular probes such as antibodies, DNA, MHC-tetramers, intracellular reporter molecules to address fundamental questions relating to the pathogenesis, treatment and complications of type 1 and 2 diabetes. The Core will be located at the Barbara Davis Center and manned by 2 FTE technical staff under the director ship of Dr. D. Bellgrau. It will use items of large equipment recently obtained from NIH and charitable funding. Twelve services will be offered including standard FACS analyses and immunohistochemistry of solid tissues and more sophisticated analyses of signal transduction and subcellular events in living cells. Formal training will be offered without charge on a biweekly to monthly basis and expert consultation will be available for each of the services provided.