CORE--MOLECULAR BIOLOGY FACILITY

描述(摘自申请)分子生物学核心设施由三个组成部分和一个由Kasahara博士指导的子核心组成，Kasahara博士将监督基于病毒的基因输送系统的产生。分子生物学核心设施支持三大类活动。第一类主要的活动集中在开发特定的试剂来表达感兴趣的cDNA。我们是一个校园范围内的非洲爪哇卵母细胞制备资源，用于基于RNA的表达研究和重组蛋白的细菌表达。我们已经增加了杆状病毒的表达，并正在为哺乳动物细胞系开发可诱导的调控表达系统。此外，我们还计划了一个由新的联合主任领导的新的分核心，他将指导基于病毒的基因输送系统的开发。该组件还执行支持上面列出的不同表达系统所需的所有子克隆活动。第二个组成部分是分子生物学指导和资源中心，介绍和指导没有经验或经验有限的研究人员利用分子生物技术。这些服务包括搜索数据库中的序列同源性，并设计用于聚合酶链式反应扩增和DNA测序的引物。分子生物学核心设施的第三个组成部分是常规分子生物学研究中所需的尖端设备的操作管理。

DESCRIPTION (Taken from the application) The Molecular Biology Core facility is composed of three components plus a sub-core directed by Dr. Kasahara, who will supervise generation of viral based gene delivery systems. The Molecular Biology Core facility supports three major classes of activities. The first major class of activity is focused on developing specific reagents to express cDNAs of interest. We are a campus wide resource for preparation of Xenopus laevis oocytes for RNA based expression studies and bacterial expression of recombinant proteins. We have added baculovirus expression and are developing inducible regulated expression systems for mammalian cell lines. In addition, we have planned a new sub-core directed by the new Co- Director, who will direct development of viral based gene delivery systems. This component also performs all the subcloning activities required to support the different expression systems listed above. The second component is a Molecular Biology Guidance and Resource Center, which introduces and guides investigators in utilizing molecular biological techniques who have no or limited experience. Included in these services are searching databases for sequence homologies and designing primers for PCR amplification and DNA sequencing. The third component of the Molecular Biology Core facility is operational management of sophisticated equipment needed in routine molecular biological studies.