CORE--ADVANCED CELL TECHNOLOGIES

描述（改编自应用程序）该核心建立于 上次竞争续订的时间。这个核心将帮助调查人员 离体维持高度分化的细胞和组织。具体 目标是提供：1.具有最先进的专门知识和设备的设施 用于进行复杂形式的细胞培养; 2.储存的高 分化的细胞系; 3.胶原酶、血清等检测试剂 测试对肝脏和GI组织的功效; 4.细胞外制剂 富含ECM的组织提取物的基质组分和/或辅助 利用市售形式的ECM组件; 5.协商 并协助设计离体培养的最佳培养条件 维持分化的细胞类型，无论是原代细胞还是细胞系; 6. 无血清的化学和微生物确定的培养基， 肝或GI组织来源的细胞或细胞系的分化; 7. 从正常或患病的肝脏组织新鲜分离的细胞悬液，或 胃肠道;和8.流式细胞术的复杂形式的援助， 来源于肝脏或GI的细胞的分离和/或表征 组织.

DESCRIPTION (Adapted from the application) This core was established at the time of the last competing renewal. This core will help the investigators with ex vivo maintenance of highly differentiated cells and tissues. The specific aims are to provide: 1.a facility with state-of-the-art expertise and equipment with which to perform sophisticated forms of cell culture; 2. stocks of highly differentiated cell lines; 3. tested reagents such as collagenase and serum tested for efficacy on liver and GI tissues; 4. preparations of extracellular matrix components of tissue extracts enriched in ECM and/or assistance with utilization of commercially available forms of ECM components; 5. consultation and assistance with designs for optimal culture conditions for ex vivo maintenance of differentiated cell types whether primary or for cell lines; 6. serum-free chemically and hormonally defined media tailored for growth or for differentiation of liver or GI tissue-derived cells or for cell lines; 7. freshly isolated cell suspensions from normal or diseased tissues of liver or GI tract; and 8. assistance with sophisticated forms of flow cytometry for isolation of and/or characterization of cells derived from the liver or GI tissue.