Core--Primate facility

该计划项目的长期目标是开发疫苗策略和免疫预防，通过将被动免疫与人类中和单抗的协同组合与主动免疫与DNA主/蛋白质增强策略相结合来防止艾滋病毒C分支在产程中的传播。该核心的总体目标是提供必要的实验动物和必要的支助服务，以促进完成项目2(被动和主动免疫)和项目3(DNA Prime/蛋白质增强疫苗接种)中概述的目标。这将包括提供来自Yerkes猕猴繁殖群体的新生和幼年无逆转录病毒(SIV/STLV-1，SRV)猕猴；口腔猕猴和艾滋病毒猕猴；用DNA或蛋白质免疫原对实验动物进行免疫；单独使用单抗或与主动免疫结合使用；对免疫动物进行病毒挑战；对实验动物进行日常监测；定期对实验动物进行体格检查和血液采集，进行血象和免疫学评估，并运往Ruprecht博士和Rasmussen博士的实验室进行体外研究；CBCs和流式细胞仪评估以确定淋巴细胞亚群；以及在研究过程中死亡或牺牲的所有实验动物的完整大体和组织学评估的表现。提供这些资源和支持服务将促进本计划项目中提出的艾滋病免疫战略的开发和测试。这些研究还将记录编码艾滋病毒C分支env基因的猿猴-人类免疫缺陷嵌合病毒的传染性和致病性，并将能够评估疫苗在预防感染(消毒免疫)或改变攻击后病毒载量方面的效力。

The long-range goal of this Program Project is to develop vaccine strategies and immunoprophylaxis against intrapartum transmission of HIV clade C by combining passive immunization with synergistic combinations of human neutralizing monoclonal antibodies with active vaccination with a DNA prime/protein boost strategy. The overall objectives for this Core are to provide the required experimental animals and support services needed to facilitate completion of the objectives outlined in Project 2 (Passive and Active Immunization) and Project 3 (DNA Prime/Protein Boost Vaccination). This will include provision of newborn and juvenile retrovirus-free (SIV/ STLV-1, SRV) rhesus macaques from the Yerkes macaque breeding colonies; oral and HIV macaques; immunizations of the experimental animals with DNA or protein immunogens; use of monoclonal antibodies alone or in combination with active immunization; viral challenge of the immunized animals; daily monitoring of the experimental animals; periodic physical examinations of the experimental animals and blood collections for hemogram and immunologic evaluations and for shipment to Drs. Ruprecht and Rasmussen's laboratories for in vitro studies; performance of CBCs and flow cytometry evaluations to determine lymphocyte subsets; and the performance of complete gross and histologic evaluation of all experimental animals that die or that are sacrificed during the course of the study. Provision of these resources and support services will facilitate the development and testing of the AIDS immunization strategies proposed in this Program Project. These studies will also document the infectivity and pathogenicity of the chimeric simian-human immunodeficiency virus encoding the env gene of HIV clade C, and will allow an assessment of vaccine efficacy with respect to either the prevention of infection (sterilizing immunity) or modification of post challenge virus load.