HOPKINS DK CENTER FOR THE ANALYSIS OF GENE EXPRESSION

描述(应用程序摘要) 基因组技术的最新进展使同时 多基因多基因表达模式的研究 生物系统。通常被称为“芯片技术”的主要方法 涉及生产包含以下物质的高密度阵列(微阵列) 之前分离的数千个互补DNA(CDNA)序列，其中 用来分析全球范围内的基因表达。潜在的 获得重要的和新的信息，以了解 这项技术的生物学和病理生物学是巨大的。我们的小组是一个 研究病毒生物学和病理生物学的研究人员组成的联盟 内分泌、肾脏或胃肠系统。以确保我们的研究 受益于快速发展的基因组技术的力量，我们建议 成立霍普金斯DK基因表达分析中心。的目标 这项研究的应用是：(1)进行全基因组的基因图谱 利用基因芯片表达所涉及的项目，以及(2) 建立一种潜在的重要和强大的主题方法 相关项目间的基因表达分析。其具体目的是 在约翰霍普金斯大学学院建立一个核心研究机构 支持基因芯片生产和基因分析的医学 调查人员的个人资料，其研究是基于 NIDDK的科学任务。奖助金申请的初始阶段 将包括15个来自现有调查人员的项目和7个试行/可行性项目 初级调查员的项目。这些项目共享一个方案 侧重于分子调控机制的研究。 肾脏、消化和内分泌器官的生物学和病理生物学。这个 项目可进一步分为三大主题，包括：(1) 基因组对应激或炎症的反应，(2)调节 上皮细胞的增殖和/或分化表型，以及(3) 糖尿病和其他代谢/内分泌的细胞和分子功能障碍 精神错乱。对阵列数据的分析将由生物信息学提供支持 以及一个生物统计核心，由各自领域的当地专家组成。 因此，此应用程序代表了一种合理且集中的方法，以 每个调查员解决的具体问题。获得的潜力 对导致这两种疾病的分子机制的新颖和相关的见解 通过使用DK进行的拟议研究的三个主要主题 由参与调查人员组成的中心是相当可观的。

DESCRIPTION (Abstract of the application) Recent advances in genomic technologies have made possible the simultaneous examination of the expression patterns of a large number of genes in many biological systems. The principal approach, often called the "chip technology" involves the production of high-density arrays (microarrays) that contain thousands of previously isolated complementary DNA (cDNA) sequences and which are used to analyze gene expression on a global scale. The potential for gaining important and novel information on the fundamental mechanisms of biology and pathobiology with this technology is enormous. Our group is a consortium of investigators who are studying the biology-and pathobiology of the endocrine, renal or gastrointestinal system. To ensure that our research benefits from the power of rapidly advancing genomic techniques, we propose to form the Hopkins DK Center for the Analysis of Gene Expression. The Goals of this research application are (1) to perform genome-wide patterning of gene expression for the involved projects using cDNA microarrays, and (2) to establish a potentially important and powerful thematic approach to the analysis of gene expression among the concerned projects. The Specific Aim is to establish a core research facility at the Johns Hopkins University School of Medicine to support the production of cDNA microarrays and the analysis of gene profiles for investigators whose research is based on areas within the scientific mission of the NIDDK. The initial phase of the grant application will include 15 projects from established investigators and 7 pilot/feasibility projects from junior investigators. These projects share a programmatic emphasis on research that is focused on the molecular regulatory mechanisms in the biology and pathobiology of the renal, digestive, and endocrine organs. The projects can further be divided into 3 major themes that include: (1) The genomic responses to stress or inflammation, (2) Mechanisms regulating the proliferative and/or differentiated phenotype in epithelial cells, and (3) Cellular and molecular dysfunction in diabetes and other metabolic/endocrine disorders. The analysis of the array data will be supported by a Bioinformatics and a Biostatistics Core, staffed by local experts in the respective field. Thus, this application represents a rational and focused approach to the specific questions that each investigator addresses. The potential for gaining novel and relevant insights into the molecular mechanisms responsible for each of the three main themes of the proposed research through the use of the DK Center by the participating investigators is considerable.