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HOPKINS DK CENTER FOR THE ANALYSIS OF GENE EXPRESSION

霍普金斯 DK 基因表达分析中心

基本信息

批准号：
6524336
负责人：
GREGORY G. GERMINO
金额：
$ 57.23万
依托单位：
JOHNS HOPKINS UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2000
资助国家：
美国
起止时间：
2000-09-30 至 2004-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6524336
关键词：
biomedical resource biotechnology cell differentiation cell proliferation clinical research complementary DNA diabetes mellitus genetics epithelium gene expression human subject inflammation informatics laboratory mouse laboratory rat microarray technology statistics /biometry stress

项目摘要

DESCRIPTION (Abstract of the application) Recent advances in genomic technologies have made possible the simultaneous examination of the expression patterns of a large number of genes in many biological systems. The principal approach, often called the "chip technology" involves the production of high-density arrays (microarrays) that contain thousands of previously isolated complementary DNA (cDNA) sequences and which are used to analyze gene expression on a global scale. The potential for gaining important and novel information on the fundamental mechanisms of biology and pathobiology with this technology is enormous. Our group is a consortium of investigators who are studying the biology-and pathobiology of the endocrine, renal or gastrointestinal system. To ensure that our research benefits from the power of rapidly advancing genomic techniques, we propose to form the Hopkins DK Center for the Analysis of Gene Expression. The Goals of this research application are (1) to perform genome-wide patterning of gene expression for the involved projects using cDNA microarrays, and (2) to establish a potentially important and powerful thematic approach to the analysis of gene expression among the concerned projects. The Specific Aim is to establish a core research facility at the Johns Hopkins University School of Medicine to support the production of cDNA microarrays and the analysis of gene profiles for investigators whose research is based on areas within the scientific mission of the NIDDK. The initial phase of the grant application will include 15 projects from established investigators and 7 pilot/feasibility projects from junior investigators. These projects share a programmatic emphasis on research that is focused on the molecular regulatory mechanisms in the biology and pathobiology of the renal, digestive, and endocrine organs. The projects can further be divided into 3 major themes that include: (1) The genomic responses to stress or inflammation, (2) Mechanisms regulating the proliferative and/or differentiated phenotype in epithelial cells, and (3) Cellular and molecular dysfunction in diabetes and other metabolic/endocrine disorders. The analysis of the array data will be supported by a Bioinformatics and a Biostatistics Core, staffed by local experts in the respective field. Thus, this application represents a rational and focused approach to the specific questions that each investigator addresses. The potential for gaining novel and relevant insights into the molecular mechanisms responsible for each of the three main themes of the proposed research through the use of the DK Center by the participating investigators is considerable.

描述（申请摘要）基因组技术的最新进展使得同时检查许多基因的表达模式生物系统。主要方法，通常称为“芯片技术” 涉及高密度阵列（微阵列）的生产，其中包含数千个先前分离的互补 DNA (cDNA) 序列，其中用于分析全球范围内的基因表达。的潜力获得有关基本机制的重要而新颖的信息生物学和病理生物学用这种技术是巨大的。我们的小组是一个正在研究生物学和病理学的研究人员联盟内分泌、肾脏或胃肠系统。为了确保我们的研究受益于快速发展的基因组技术的力量，我们建议组建霍普金斯 DK 基因表达分析中心。的目标该研究应用是（1）对基因进行全基因组模式分析使用 cDNA 微阵列表达所涉及的项目，以及 (2) 建立一个潜在重要且有力的主题方法相关项目的基因表达分析。具体目标是在约翰·霍普金斯大学医学院建立核心研究设施支持 cDNA 微阵列生产和基因分析的药物其研究基于该领域内的研究人员的概况 NIDDK 的科学使命。拨款申请的初始阶段将包括来自既定研究人员的 15 个项目和 7 个试点/可行性项目初级研究人员的项目。这些项目共享一个程序化的重点关注分子调控机制的研究肾脏、消化和内分泌器官的生物学和病理学。这项目可进一步分为 3 个主要主题，包括： (1) 基因组对应激或炎症的反应，(2) 调节机制上皮细胞的增殖和/或分化表型，以及（3）糖尿病和其他代谢/内分泌的细胞和分子功能障碍失调。阵列数据的分析将得到生物信息学的支持以及生物统计核心，由各自领域的当地专家组成。因此，该应用程序代表了一种合理且有针对性的方法每个研究者要解决的具体问题。获得的潜力对负责每个的分子机制的新颖且相关的见解通过使用 DK 提出的研究的三个主题中心参与调查的人员相当多。