REGULATION OF ENERGY METABOLISM IN MOSQUITOES

描述：雌性蚊子需要脊椎动物宿主的血液， 产蛋。蚊子对公众健康的重要性源于这样一个事实， 当蚊子从人类宿主身上获得这种血餐时， 传播疾病，如疟疾和登革热，这在世界范围内是 每年造成数百万人死亡我们需要大幅增加 我们对蚊子生物学的理解。一个地区， 到目前为止，被忽视的是基础代谢研究， 了解雌蚊如何利用血液中的成分 餐最多20%的血粉氨基酸用于鸡蛋 生产和这个建议的目的是决定其他的命运 百分之八十因此，该建议涉及以下命运氨基 在各种营养状态下从血粉蛋白质衍生的酸。 此外，我们建议评估现有能源储备在以下方面的作用： 雌蚊在吸血过程中。一旦相关途径 已经定义，未来的研究将关注的机制， 在不同的营养、行为和环境条件下调节途径 条件在这个建议中，我们计划定量地研究 来源于埃及伊蚊（Aedes aegypti）的血粉蛋白的氨基酸， 登革热和疟疾媒介-白色按蚊 美国参考 提出了以下具体目标：1。确定代谢的命运 14 C标记的血粉蛋白质氨基酸。2.确定代谢的命运 预先存在的能量储存，特别是糖原和脂质， 餐3.表征血粉蛋白质氨基酸的代谢。

DESCRIPTION: Female mosquitoes require a blood meal from a vertebrate host to produce eggs. The public health importance of mosquitoes derives from the fact that while obtaining such a blood meal from a human host the mosquito is able to transmit diseases, such as malaria and dengue, which world-wide are responsible for millions of deaths per year. We need to significantly increase our understanding of the biology of mosquitoes. One area that has been neglected, so far, is fundamental metabolic studies directed towards understanding how the female mosquito utilizes the components of the blood meal. At most 20 percent of the blood meal amino acids are used for egg production and the purpose of this proposal is determine the fate of the other 80 percent. Thus, this proposal is concerned with following the fate amino acids derived from blood meal proteins under a variety of nutritional states. In addition, we propose to assess the role of preexisting energy reserves in the female mosquito during blood meal utilization. Once the relevant pathways have been defined, future studies will be concerned with mechanisms that regulate the pathways under different nutritional, behavioral and environmental conditions. In this proposal we plan to quantitatively examine the fate of amino acids derived from blood meal proteins in Aedes aegypti, the vector of dengue fever and Anopheles albimanus, a vector of malaria in Central and South America. The following specific aims are proposed: 1. Determine the metabolic fate of 14C-labeled blood meal protein amino acids. 2. Determine the metabolic fate of preexisting energy stores, specifically glycogen and lipid, following a blood meal. 3. Characterize the metabolism of blood meal protein amino acids.