TRYPANOSOMA CRUZI-INDUCED HOST CELL SIGNALING RESPONSES

描述（改编自申请人的摘要）：信号转导 在宿主细胞中由T. cruzi 是入侵过程所必需的。特别是第二信使Ca+2 和cAMP是T. Cruzi诱导宿主细胞信号传导。的目标 这项建议的目的是阐明这些信号通路， 鉴定所涉及的特定宿主蛋白因子。主机 细胞激酶和磷酸酶将被确定，它们在入侵中的作用 和/或宿主细胞基因表达。由下列因素引起的事件 野生型里氏木cruzi将与由突变株诱导的那些进行比较， 在触发特定的Ca+2宿主细胞反应方面有缺陷， 哺乳动物细胞的入侵。最后，cDNA微阵列杂交将是 用于鉴定在T. 克氏感染

DESCRIPTION (Adapted from the Applicant's Abstract): Signal transduction pathways triggered in host cells by the mammalian-infective forms of T. cruzi are required for the invasion process. Specifically the second messengers Ca+2 and cAMP are known effectors of T. cruzi induced host cell signaling. The goals of this proposal are to elucidate these signaling pathways by the identification of the specific host protein factors that are involved. Host cell kinases and phosphatases will be identified and their roles in invasion and/or host cell gene expression will be characterized. The events induced by wild-type T. cruzi will be compared to those induced by a mutant strain that is defective in triggering a specific Ca+2 host cell response, required for invasion of mammalian cells. Finally, cDNA micro array hybridization will be used to identify host cell genes that are differentially expressed during T. cruzi infection.