CORE--GENERAL SCIENTIFIC

一般科学核心的目的是协助研究人员在动物外科，免疫细胞化学，透射电子显微镜和图像分析要求的程序项目教师。核心还协助研究人员进行各种生化分析，以及分子生物学方法。核心设施包括进行拟议研究所需的人员和设备。该核心将进行一般的动物准备，包括麻醉、气管切开、置管、体温监测和生理变量的调节。如个别项目所述，Core将协助研究人员为创伤性脑损伤的动物做准备。此外，还可以利用专门知识修改实验准备，以便将来的研究需要。核心人员将进行免疫细胞化学程序，以及开发新的程序，以显示额外的抗体。Core将处理用于塑料嵌入的组织，用于光镜和电子显微镜分析。高质量的薄片将由核心人员制作，并染色用于超微结构分析。已建立的图像分析程序将用于定量免疫反应细胞的频率，以及确定挫伤区域和体积。最后，核心将协助调查人员在暗室程序和生成的摄影幻灯片和印刷品。

The purpose of the General Scientific Core is to assist investigators in animal surgery, immunocytochemistry, transmission electron microscopy, and image analysis requirements for the Program Project faculty. The Core also assists investigators in various biochemical analyses, as well as molecular biological approaches. The Core facilities consists of personnel and equipment needed to perform the proposed studies. This Core will conduct general animal preparation, including anesthesia, tracheotomy, catheterization, temperature monitoring, and regulation of physiological variables. The Core will assist investigators in preparing the animals for traumatic brain injury, as described in the individual projects. In addition, expertise is available to modify experimental preparations that may be required for future investigations. Core personnel will carry out immunocytochemical procedures, as well as develop new procedures for the visualization of additional antibodies. The Core will process tissues for plastic embedding for both light and electron microscopic analysis. High quality thin sections will be produced by personnel within the Core and stained for ultrastructural analysis. Established image analysis procedures will be utilized to quantitate the frequency of immunoreactive cells, as well as to determine contusion areas and volume. Finally, the Core will assist investigators in darkroom procedures and the generation of photographic slides and prints.