MODULATION OF NEOCORTICAL INTERNEURONAL FUNCTION

描述：(改编自《调查者摘要》)：Ascending 脑干和基底前脑的神经递质系统调节 直接作用于GABA能抑制的大脑皮层网络活动 中间神经元。数据表明，神经递质，如乙酰胆碱(ACh) 5-羟色胺(5-羟色胺)可以选择性地激活特定的亚群 通过不同类型的突触后受体和 从而改变大脑皮层回路中的信息流。这些实验 将检查ACh和5-羟色胺对低阈值棘波(LTS)和FAST的影响 大鼠视皮层V层的GABA能中间神经元。关于 膜片钳技术将用于获得全细胞记录以评估 不同亚类胆碱能和5-羟色胺能受体的亚型 中间神经元和突触后反应的性质。成对的 记录，单一抑制性突触后电流将被诱发 锥体神经元与胆碱能和5-羟色胺能突触前效应 将对激动剂进行评估。生物细胞素联用于细胞内标记 将使用钙结合蛋白和神经肽的免疫细胞化学 以及电生理行为来对中间神经元进行分类，以进一步 检验中间神经元亚群被选择性地调制的假设 ACh和5-羟色胺。鉴于GABA能抑制网络在 信息处理和病理生理过程，如癫痫的发生， 观察到这些上升的神经递质系统可能是 皮质损伤后的解剖学改变，有关行动的信息 像ACh和5-羟色胺这样的调节剂对我们理解正常 和紊乱的皮质功能。

DESCRIPTION: (Adapted from the Investigator's Abstract): Ascending neurotransmitter systems from the brainstem and basal forebrain regulate activities in cortical networks through direct actions on GABAergic inhibitory interneurons. Data suggest that neurotransmitters such as acetylcholine (ACh) and serotonin (5-HT) can selectively activate particular subgroups of inhibitory interneurons through different classes of postsynaptic receptors and thus modify the flow of information in cortical circuits. These experiments will examine effects of ACh and 5-HT on low threshold spike (LTS) and fast spiking aboutS) GABAergic interneurons in layer V of rat visual cortex. about Patch-clamp techniques will be used to obtain whole cell recordings to evaluate the subtypes of cholinergic and serotonergic receptors on different subclasses of interneurons and the nature of the postsynaptic responses. In paired recordings, unitary inhibitory postsynaptic currents will be evoked on pyramidal neurons and presynaptic effects of cholinergic and serotonergic agonists will be assessed. Intracellular labeling with biocytin combined with immunocytochemistry for calcium binding proteins and neuropeptides will be used along with electrophysiological behavior to classify interneurons to further test the hypothesis that subgroups of interneurons are selectively modulated by ACh and 5-HT. Given the importance of the GABAergic inhibitory network in information processing and pathophysiologic processes such as epileptogenesis, and the observation that these ascending neurotransmitter systems may be altered anatomically following cortical lesions, information regarding action of modulators like ACh and serotonin is critical to our understanding of normal and disordered cortical function.