Core--DNA microarray & homology modeling

DNA微阵列和同源建模核心将具有几个主要功能。首先，它将成为实验室研究接触有毒物质如何影响基因表达的资源。实验室将与核心人员(项目负责人、高级调查人员或PGR)讨论计划中的实验，以便制定切实可行的计划。这样的讨论将导致讨论是否最佳地解决给定的问题(A)通过消减杂交产生有限的cDNA阵列，(B)使用Core储存库中可用的cDNA子集，或(C)通过储存库中可用的完整的cDNA阵列。当减法杂交阵列被认为是合适的方法时，核心将通过代表性差异分析来帮助其生成，这是Gregg博士(高级调查员)拥有丰富经验的一种技术。随着Core扩大其cdna克隆库，预计重点将逐渐转移到更大的阵列上。尤其是在人类基因的情况下，通过吴博士(高级研究员)和同事的影响，我们已经拥有了近10,000个克隆。Core将为Superfund实验准备阵列，进行杂交和扫描，将结果提交给实验室并帮助解释。核心的第二个主要功能将是三维同源建模。这将允许超级基金调查人员以电子方式检查蛋白质与毒物的相互作用，从而获得可应用于其他潜在目标的有价值的机械信息。核心的第三个主要结合点是生物信息学。这将主要包括数据管理，协助超级基金调查人员进行数据分析和数据库搜索，并帮助调查人员利用校园生命科学信息学计划。初始数据处理将在此核心中进行。随着数据集变得更大，它们将被转移到统计核心B。

The DNA Microarray and Homology Modeling Core will have several major functions. First it will be a resource for laboratories to examine how exposure to toxic agents affects gene expression. Laboratories will discus with Core personnel (Project Leaders, Senior Investigators, or PGR) projected experiments so that practical plans can be developed. Such discussion will lead to discussions whether a given problem is addressed best (a) by generating a limited cDNA array by subtractive hybridization, (b) using a subset of cDNAs available in the Core repository, or (c) through a full array of cDNAs available in the repository. When a subtractively hybridized array is considered the appropriate approach, the Core will assist in its generation by representational difference analysis, a technique with which Dr. Gregg (Senior Investigator) has much experience. As the Core expands its repository of cDNA clones, the emphasis is anticipated to shift gradually to larger arrays. This will be true particularly in the case of human genes, for which we already have at our disposal nearly 10,000 clones through the effects of Dr. Wu (Senior Investigator) and colleagues. The Core will prepare arrays for Superfund experiments, conduct the hybridization and scanning, present the results to the labs and help with the interpretations. A second major function of the Core will be in 3-D homology modeling. This will permit Superfund investigators to examine protein-toxicant interactions electronically, thereby gaining valuable mechanistic information that can be applied to other potential targets. The third major junction of the Core is in bioinformatics. This will consist largely of data management, assisting Superfund investigators with data analysis and database searchers and helping investigators utilize the campus Life Sciences Informatics Program. Initial data handling will be in this core. As data sets become larger they will be transferred to the Statistics Core B.