Interaction Of Pathogenic Bacteria With Leukocytes

项目目标和目的：具体项目将1)确定和表征病原菌逃避或颠覆正常吞噬细胞反应从而导致疾病的特定机制，以及2)调查人类对病原菌的吞噬细胞反应机制。研究成果：1)我们已经确定A组链球菌分泌人CD11b的同源物，阻断先天和获得性免疫功能。这代表了A组链球菌在人类感染过程中存活的一种新机制。2)我们使用人类寡核苷酸芯片(Affymetrix GeneChip)来检测受体介导的吞噬过程中人中性粒细胞的整体基因表达谱。我们发现吞噬后的基因转录主要决定PMN的命运。我们确定了激活的人中性粒细胞和正在发生自发凋亡的人的完整的凋亡途径(&gt；100基因)。这些研究将被用作研究人类病原体的跳板。

Project Goals and Objectives: Specific projects will 1) identify and characterize specific mechanisms used by pathogenic bacteria to evade or subvert normal phagocyte responses and therefore cause disease, and 2) investigate human phagocyte response mechanisms to pathogenic bacteria. Research Accomplishments: 1) We have determined that Group A Streptococcus secretes a homologue of human CD11b that blocks innate and acquired immune function. This represents a novel mechanism by which Group A Streptococcus can survive during the course of human infection. 2)We used human oligonucleotide microarrays (Affymetrix GeneChip) to determine global gene expression profiles in human PMNs during receptor-mediated phagocytosis. We discovered that gene transcription following phagocytosis serves mainly to determine PMN fate. We identified entire apoptotic pathways (>100 genes) for activated human PMNs and for those undergoing spontaneous apoptosis. These studies will be used as a springboard for studies with human pathogens.