Dynamic structural study of scaffolding proteins FtsZ and MipZ in Rhodobacter sphaeroides using ultra-high spatial-temporal resolution structured illu

本项目致力于开发一种新型的活细胞成像显微镜工具，该工具结合了固体浸没透镜和结构照明显微镜。人们认为，这项技术将提供改进的空间和时间分辨率，从而更好地了解细胞组织，特别是像细菌这样小的生物体。该项目还将采用其他成像技术，如3D光激活定位显微镜、旋转盘共聚焦显微镜超分辨率径向波动和单粒子跟踪，以补充正在开发的主要技术。这种成像方法将在球形红杆菌上进行测试，这是一种在阿米蒂奇实验室广泛研究的革兰氏阴性紫色细菌。重点将是成像在细胞分裂中使用的两个关键蛋白质；自贸区和保税区。两者都在细胞分裂前形成聚合物环，然而，这些环相对于彼此的确切行为和位置不能用现有的技术来解决。WCUB, ENWW

This project focuses on developing a novel microscopy tool for live cell imaging which combines a Solid Immersion Lens with Structured Illumination Microscopy. It is thought that this technique will provide improved spatial and temporal resolution allowing greater understanding of cellular organisation, particularly in organisms as small as bacteria. The project will also employ other imaging techniques such as 3D Photo-activated Localisation Microscopy, Spinning Disk Confocal Microscopy Super Resolution Radial Fluctuations and Single Particle Tracking to compliment the primary technique being developed.This imaging approach will be tested on Rhodobacter sphaeroides, a gram negative purple bacterium which is widely studied in the Armitage Lab. The focus will be to image two key proteins employed in cell division; FtsZ and MipZ. Both form polymer rings at the divisome of the cell prior to division, however the exact behaviour and position of these rings relative to each other cannot be resolved using techniques currently available. WCUB, ENWW