PROPERTIES OF LYMPHOID TUMOR CELLS IN VIVO AND IN VITRO

宿主T细胞生发中心(GC)与B淋巴瘤细胞的相互作用 将会被检查。需要回答的一个关于 SJL小鼠淋巴肿大：MTV-29高编码的原因 VSAg转录以及它与这些基因的GC来源有何关系 淋巴瘤(RCS)？MTV-29在肝脏和RCS DNA中的5‘LTR将是 测序以确定5‘LTR是在所有细胞中都有缺陷还是只在细胞中有缺陷 在RCS细胞中解释vSAg起始点在终止区 MTV-29。该vSAg表达的调节将通过使用 报告结构的横切和寻找特定的结合因子 对于MTV-29中的区域，在RCS和正常GC中表达，但在其他 B细胞。体内促进生长的机制还有待研究， 包括vbeta16+T细胞在c57L小鼠中的命运将被分析。这个 Vbeta16 T细胞在发育期原发疾病小鼠外周血中的表达 淋巴瘤及抗vbeta16抗体对肿瘤生长的影响 活体评估。将尝试确定继发性致癌因素 导致持续扩散和缺乏差异化的事件 E 淋巴瘤细胞。将确定RCS、正常分离GC NJ101细胞对自发激素和糖皮质激素的耐受性不同 诱导细胞凋亡。几种癌基因在形态上的表达 BCL-2转基因小鼠和正常SJL小鼠的异常和正常GC将 要好好学习。来评估~逆转免疫现象是否 监测~可能适用于人类，CD4+T细胞渗入 人类GC来源的淋巴瘤将接受Vbeta或Va TCR检查 可能反映对特定抗原或vβ的单克隆反应的模式 对SAG的反应受限。

The host T cell germinal center (GC) derived B lymphoma cell interaction will be examined. A central question to be answered about the lymphomagenesis in SJL mice is: What causes the high mtv-29 encoded vSAg transcription and how does it relate to the GC origin of these lymphomas (RCS)? The 5' LTR of mtv-29 in liver and RCS DNA will be sequenced to determine whether the 5'LTR is defective in all cells or only in RCS cells to explain that the vSAg initiation site is in the end region mtv-29. Regulation of the expression of this vSAg will be studied by using transection of reporter constructs and looking for specific binding factors for regions in mtv-29, expressed in RCS and normal GC but not in other B cells. The mechanism of the growth promotion in vivo will be studies, including the fate of vbeta16+ T cells in c57L mice will be analyzed. The vbeta16 T cell representation in PBL of mice with developing primary lymphomas will be followed and the effect of anti-vbeta16 on tumor growth in vivo evaluated. Attempts will be made to identify secondary oncogenic events that cause continued proliferation and a lack of differentiation in e lymphoma cells. It will be determined whether RCS, normal isolated GC and NJ101 cells differ in resistance to spontaneous and glucocorticosteroid induced apoptosis. The expression of several oncogenes in morphologically abnormal and normal GCs from bcl-2 transgenic and normal SJL mice will be studies. To evaluate whether the phenomenon of ~reverse immune surveillance~ may be applicable to humans, CD4+ T cell infiltrates in human GC-derived lymphomas will be examined for Vbeta or Va TCR patterns that might reflect monoclonal responses to specific Ag or v beta restricted responses to a SAg.