MOLECULAR AND CELLULAR STUDIES OF CIRCADIAN RHYTHMS

总的目标是继续对果蝇的分析 利用遗传和分子方法研究昼夜节律系统。里的钟 已知果蝇是由一个反馈回路组成的， 时钟基因的协调作用-周期（per），无时间（tim），时钟（Clk）， 周期（cyc），双倍时间（dbt）。所有这些基因在哺乳动物中都是保守的， 以类似的方式（如果不是完全相同的话）调节哺乳动物的昼夜节律， 节奏该应用旨在确定新基因/突变的作用 最近在PI的实验室分离，并分离额外的基因 通过微阵列技术。此外，将使用基于dsRNA的方法 为了降低组织特异性细胞中的时钟和推定的时钟基因的活性， 方式

The overall goal is to continue the analysis of the Drosophila circadian system using genetic and molecular approaches. The clock in Drosophila is known to be composed of a feedback loop generated through the coordinated action of clock genes- period (per), timeless (tim), Clock (Clk), cycle (cyc), double-time (dbt). All of these genes are conserved in mammals and function in similar, if not identical, ways to regulate mammalian circadian rhythms. This application seeks to determine the role of new genes/mutations recently isolated in the PI's laboratory and to isolate additional genes through microarray technology. In addition, a ds-RNA-based method will be used to reduce activity of clock and putative clock genes in a tissue-specific manner.