MODULATION OF OLFACTORY BULB NEURON CURRENT PROPERTIES

(改编自《调查者摘要》)：设计的研究是一个 钾电流调制的多学科分析 嗅球的颗粒细胞和二尖瓣细胞。广泛的、长期的 本研究的目的是阐明神经促性腺激素是如何促进生长的 因子，而细胞质蛋白激酶可以利用离子通道作为 产生短期和长期磷酸化的底物 突触效能的可塑性变化或有助于建立 嗅球神经元的神经回路，Kvl.3 电生理、生化和分子方法。我们会 电生理检查电流大小和门控的变化 激活颗粒内酪氨酸磷酸化诱导的动力学 并测定二尖瓣细胞酪氨酸特异性磷酸化程度。 以克隆的Kv1.3为模型，结合生化测定 激酶诱导的酪氨酸磷酸化和分子突变将 阐明离子多重磷酸化的机制细节 频道。该提案将提供有关 An上多重磷酸化收敛的分子机制 离子通道在嗅球这一大脑区域显著表达 对于这种情况，通过磷酸化进行的神经调节完全没有描述。

(Adapted from the Investigator's Abstract): The designed research is a multidisciplinary analysis of the modulation of the potassium currents in granule and mitral cells of the olfactory bulb. The broad, long-term objective of this research is to elucidate how neurotropins, growth factors, and cytoplasmic protein kinases can utilize ion channels as substrates for phosphorylation to give rise to short-term and long-term plastic changes in synaptic efficacy or to aid in the establishment of neural circuits in the olfactory bulb neurons, Kvl.3, using electrophysiological, biochemical, and molecular approaches. We will electrophysiologically examine changes in current magnitude and gating kinetics induced by activating tyrosine phosphorylation in granule and mitral cells and measure the degree of tyrosine specific phosphorylation. By utilizing the cloned Kvl.3 as a model, combined biochemical measurement of kinase-induced tyrosine phosphorylation and molecular mutagenesis will elucidate the mechanistic details of multiple phosphorylation of the ion channel. The proposal will provide new important information regarding the molecular mechanism for the convergence of multiple phosphorylation on an ion channel prominently expressed in the olfactory bulb, a brain region for which neuromodulation by phosphorylation is completely undescribed.