Engineering Joint Scaffolds for Function/Regeneration

描述：(逐字)组织工程学为颞下颌关节(TMJ)重建提供了相当大的希望，这是一个紧迫的临床问题。至 创建耐用的工程化关节植入物，支架材料和 必须了解组织再生和功能的架构。填满 这一迫切需要，我们必须能够系统地研究受控脚手架 建筑对骨再生、骨-软骨再生的影响 承重能力。在本BRP中，我们将确定设计的 并构建了骨髓再生骨的内部结构 体内成骨模型中的基质细胞。我们将机械地测试 这些架构决定了承载能力。为了测试 在体内骨-软骨界面再生，我们将创造一个支架 骨髓基质细胞种植于兔半侧的界面设计 支架(骨侧)和另一半上的耳软骨细胞(软骨 侧面)，在支架内部形成骨-软骨界面。最后，我们 然后，我将基于MOST设计一个Conylar Ramus单元(CRU)的原型 来自骨-骨和骨-软骨支架研究的有希望的数据。这个 本BRP的主要目标是：1)确定两种支架材料如何 (羟基磷灰石(HA)和聚酸酐以及内部的四种孔隙率变化 受控结构影响骨再生和承载能力。 2)确定如何使用HA和聚酸酐设计支架界面 骨半部与聚酸酐和聚乙二醇酯对软骨半部的影响 骨-软骨界面再生3)测试一个原型CRU支架， 在活体小型猪模型中结合了来自1和2的最好结果3，6 还有12个月。原型CRU将设计好外部形状和脚手架 建筑。我们的前两个具体目标是应用基于图像的优化 设计和固体自由形式制造，以创建支架。剩下的 四个具体目标是调查这些支架的性能 机械和使用皮下模型，导致活体小型猪 原型CRU的测试。

DESCRIPTION: (Verbatim) Tissue engineering offers considerable promise for temporomandibular (TMJ) joint reconstruction, a pressing clinical problem. To create durable engineered joint implants, the effects of scaffold material and architecture on tissue regeneration and function must be understood. To fill this vital need, we must be able to systematically study controlled scaffold architecture effects on bone regeneration, bone-cartilage regeneration, and load bearing capability. In this BRP, we will determine the effects of designed and fabricated internal architectures on bone regeneration by bone marrow stromal cells in an in vivo model of osteogenesis. We will mechanically test these architectures to determine load carrying capability. To test bone-cartilage interface regeneration in vivo, we will create a scaffold interface design seeded with bone marrow stromal cells on one half of the scaffold (bone side) and auricular chondrocytes on the other half (cartilage side), creating a bone-cartilage interface inside the scaffold. Finally, we will then engineer a prototype Conylar Ramus Unit (CRU) based on the most promising data from the bone-bone and bone-cartilage scaffold studies. The primary goals of this BRP are to: 1) Determine how two scaffold materials (hydroxyapatite (HA) and polyanhydride and four porosity variations within controlled architectures affect bone regeneration and load carrying capability. 2) Determine how scaffold interface designs using HA and polyanhydride for the bone half and polyanhydride and PGA for the cartilage half affect bone-cartilage interface regeneration 3) Test one prototype CRU scaffold that incorporates the best results from 1 and 2 in an in vivo minipig model at 3, 6 and 12 months. The prototype CRU will have designed external shape and scaffold architecture. Our first two specific aims are to apply image-based optimal design and solid free-form fabrication to create the scaffolds. The remaining four specific aims are to investigate the performance of these scaffolds mechanically and using subcutaneous models, resulting in the in vivo minipig test of a prototype CRU.