CORE--IN VITRO, IN VIVO ASSAY SYSTEMS

描述(申请人提供)：体外和体内研究的重点 养鱼核心是提供试剂、专家咨询和实际服务 对于研究人员用鱼类细胞培养物或活鱼进行实验 几个物种。许多正在进行和计划中的研究项目 中心利用培养的细胞进行形态、生理、 电生理和生化研究。此核心将提供所有 建立、维护和分析细胞培养的必要资源 水生鱼类(和无脊椎动物)。这将包括以下方面的援助 开发满足专业需求的新技术以及应用 细胞维护和操作的常规程序。里面的技术员 在下列情况下，此核心还可用于设置和维护区域性 这是必要的。各种菊花细胞系和原代培养物以及 斑马鱼的原代培养物将继续提供给调查人员。 由美国典型养殖库保存的任何鱼类细胞系 还将根据本核心中的技术人员的需要获得和维护。 在这个核心的三个完整的动物设施中，柔鱼的致癌 转基因斑马鱼实验室和受控暴露系统，将 也可供中心调查人员使用。 此外，这个核心将作为获得动物的中介，持有 实验鱼孵化场的水箱和动物护理服务 RSMAS园区。此功能之前由Core 6执行，它将 在拟议的续签计划中被删除。这种资源的重新分配是 基于过去5年对Core 6实际使用级别的经验 好几年了。

Description (provided by applicant): The emphasis of the In vitro and In vivo Fish Culture Core is to provide reagents, expert advice and actual services for investigators doing experiments with fish cell cultures or live fish of several species. Many of the ongoing and planned research projects of the Center utilize cultured cells for morphological, physiological, electrophysiological and biochemical studies. This Core will provide all resources necessary for set-up, maintenance and analysis of cell cultures from aquatic fishes (and invertebrates). This will include assistance in developing new techniques for specialized needs as well as application of routine procedures for cell maintenance and manipulation. The technician in this Core will also be available to set-up and maintain cultures, when necessary. A variety of damselfish cell lines and primary cultures and zebrafish primary cultures will be continuously available to investigators. Any of the fish cell lines maintained by the American Type Culture Collection will also be obtained and maintained as needed by the technician in this Core. Within this Core three whole animal facilities, the damselfish carcinogenesis and transgenic zebrafish laboratories and the controlled exposure system, will also be made available to Center investigators. In addition, this Core will act as an intermediary to obtain animals, holding tanks and animal care services from the experimental fish hatchery on the RSMAS campus. This function was previously performed by Core 6, which will be dropped in the proposed renewal program. This reallocation of resources is based on experience with actual usage levels of Core 6 during the past 5 years.