CORE--NMR

核磁共振核心设施为该中心和其他单位提供 校园里有最先进的核磁共振波谱。核磁共振设施，目前 位于化学系的是以“开放式商店”为基础运作的。 迈克尔·斯通博士是Core的领导人，他得到了马库斯先生的协助 Voehler操作、故障排除和维护设备并培训新员工 用户。电子专家斯坦·博托姆斯先生提供的服务包括 也被利用了。中心调查人员，哈里斯博士，他有一个有影响力的 在设施运营中的角色为中心提供咨询 人事部。他和马内特博士是斯通博士的主要合作者。 常规分析以及先进的多维核磁共振技术 执行复杂的结构。目前有200 MHz、300 MHz、400 MHz 以及设施内的500 MHz仪器。500 MHz核磁共振已升级 包括3RF通道和场梯度功能，以及控制台升级 正在进行300 MHz和400 MHz仪器的测试。总共六个硅片 利用图形工作站进行光谱处理和分子传导 建模和动力学研究。双方建立了新的合作伙伴关系 范德比尔特大学医学中心，范德比尔特大学 包括文理学院、皮博迪学院和该学院 工程学院将运营一家新指定的范德比尔特大学磁力公司 共振中心(VUMRC)除了现有的设备，一个600兆赫的 800 MHz核磁共振和3特斯拉全身成像系统将是 添加到此中心。额外的技术支持，以帮助当前 核磁共振专家马库斯·沃勒将被任命。预计 斯通博士和沃勒先生将参与管理新的 大学范围内的VUMRC。在本资助期内，中心 研究人员构成了核磁共振核心的主要用途 设施。到目前为止，使用该设施最多的是DNA成员 损伤与诱变研究核心。然而，该设施也得到了服务 酶促氧化与共轭研究的核心--氧化损伤 研究核心和环境病理学研究核心。九十二 同行评议的出版物报告了从核磁共振设施获得的结果。 已有计划确定核磁共振核心设备的使用优先顺序。确实有 两种类型的用户；需要常规结构核磁共振服务的用户 证明，以及那些使用全系列2-D功能的人 生物学方面的工作。前者的工作主要是用300 MHz的核磁共振完成的， 而其他三种仪器则专门用于二维和其他先进的 技术在计划的基础上。600和800 MHz的仪器当他们 到达也将用于后一种目的。获取仪器的途径 在核心设施中通过Markus Voehler或Via进行的“动手”培训 与中心调查人员或核心人员合作。在下一笔赠款中 期间，预计环境部门将更多地使用核磁共振设备 病理学与酶的氧化和结合研究核心。《中心》 支付中心工作人员累积的小时使用费的75%，更少的是 由他们的学术单位贡献。该中心还提供部分(15%) 斯通博士的薪水是多少？S的薪水。该中心还为 购买新设备。总体而言，该中心对实验室的贡献 运营费用直接与其对 设备。

The NMR Core Facility provides the Center and other units on campus with state-of-the-art NMR spectroscopy. The NMR facility, presently located in the Chemistry Department is operated on an "open shop" basis. Dr. Michael Stone is the leader of the Core and he is assisted by Mr. Markus Voehler who operates, troubleshoots and maintains the equipment and trains new users. The services of Mr. Stan Bottoms, a staff electronics specialist are also utilized. Center investigators, Drs. Harris who has had an influential role in the operation of the facility provides consultation to Center personnel. He and Dr. Marnett are major collaborators with Dr. Stone. Routine analyses as well as advanced multi-dimensional NMR techniques for complex structures are performed. There are presently 200MHz, 300MHz, 400MHz and 500MHz instruments within the facility. The 500MHz NMR has been upgraded to include 3RF channels and field gradient capabilities, and console upgrades are underway for the 300MHz and 400MHz instruments. A total of six Silicon Graphics workstations are used to work up spectra and conduct molecular modeling and dynamics studies. A new partnership has been created between the Vanderbilt University Medical Center, Vanderbilt University Central which includes the College of Arts and Science, the Peabody College and the School of Engineering to operate a newly designated Vanderbilt University Magnetic Resonance Center (VUMRC). In addition to the existing equipment, a 600MHz and an 800MHz NMR as well as a 3 Tesla whole body imaging system will be added to this Center. Additional technical support to assist the current NMR specialist, Markus Voehler will be appointed. It is anticipated that Dr. Stone and Mr. Voehler will be involved in the management of the new University-wide VUMRC. During the present funding period, Center investigators constituted the majority of utilization of the NMR Core Facility. By far the heaviest users of the facility were members of the DNA Damage and Mutagenesis Research Core. The facility, however, also serviced the Enzymatic Oxidation and Conjugation Research Core, the Oxidative Damage Research Core, and the Environmental Pathology Research Core. Ninety-two peer-reviewed publications reported results obtained from the NMR facility. A plan exists for prioritizing the usage of the NMR Core Facility. There are two types of users; those who require routine NMR services for structure proof, and those who use the full range of 2-D capabilities for structural biology work. The work for the former is done largely with the 300 MHZ NMR, while the other three instruments are dedicated for 2-D and other advanced techniques on a scheduled basis. The 600 and 800 MHZ instruments when they arrive will also be used for the latter purposes. Access to the instruments in the Core Facility are through "hands on" training by Markus Voehler or via collaboration with Center investigators or Core personnel. In the next grant period heavier use of the NMR Facility is expected from the Environmental Pathology and Enzymatic Oxidation and Conjugation Research Cores. The Center contributes 75% of the hourly use charges accrued by Center personnel, less is contributed by their academic units. The Center also provides a portion (15%) of Dr. Stone?s salary. The Center has also contributed considerably to the purchase of new equipment. Overall, the Center contribution to the laboratory operating expenses is directly in proportion of its utilization of the equipment.