CORE--ANALYTICAL CYTOMETRY AND IMAGE ANALYSIS FACILITY

通过此共享设施，中心成员可以访问 高质量的细胞和图像分析仪器和技术 服务。仪器包括：(1)库尔特史诗精英流 细胞仪/细胞分选仪：(2)Coulter EPICS Profile II流式细胞仪； (3)Becton-Dickenson FACSCAN；(4)子午线ACAS 570图像分析系统； (5)子午线透视激光扫描共聚焦显微镜。这些乐器 为研究人员提供细胞、颗粒和 组织样本。调查人员可以让设施工作人员分析样本，或者 能够与技术专家合作分析样本和结果数据。 《核心》由黛布拉·拉斯金博士执导，隶属于 EOHSI和CINJ的行政控制。咨询委员会召开会议 每两年一次，或根据需要，与拉斯金博士和尤尔科博士(副主任 设施核心)以审查运营、使用优先级政策和未来 计划。 优先权是由D.Laskin博士和E.Yurkow博士设定的，并基于自然 所需的项目和仪器设备。用户可以访问 只要尤尔科医生或技术人员对他们进行培训，仪器就可以24小时全天候使用。 使用该设施的费用是根据人员时间(包括 设置)和用品成本。EHS中心支持资金、关键人员和 支付设备维护费用。向中心会员收取的费用是 比其他学术用户低50%到75%。 中心成员占所有用户的30%-50%；约25名EHS中心成员，来自 所有四个研究核心都使用了该设备。给出了几个例子 突出该设施对中心的重要性的项目S 跨学科研究。该设施最近进行了扩展，增加了新的 仪器和现有装置的大修。 未来计划包括：(1)升级子午线ACAS系统光学系统和 计算机；(2)用新的Coulter Profile II替换 乐器；以及(3)扩展现有的迷你课程和实践教程 采用流式细胞术和图像分析的方法。

This shared facility affords members of the Center access to high quality cytometry and image analysis instrumentation and technical services. Instrumentation includes: (1) Coulter EPICS Elite flow cytometer/cell sorter; (2) Coulter EPICS Profile II flow cytometer; (3) Becton-Dickenson FACSCAN; (4) Meridian ACAS 570 image analysis system; and (5) Meridian Insight laser scanning confocal microscope. These instruments provide investigators with multiparameter analyses of cells, particles and tissue samples. Investigators can have samples analyzed by Facility staff, or can work with technical experts to analyze samples and resultant data. The Core is directed by Dr. Debra Laskin and is under the shared administrative control of EOHSI and CINJ. An advisory committee meets biannually, or as needed, with Drs. Laskin and Yurkow (Associate Director of the Facility Core) to review operations, usage priority policies and future plans. Priorities are set by Drs. D. Laskin and E. Yurkow and are based on the nature of the project and instrumentation required. Users can have access to instruments 24 hr per day, once Dr. Yurkow or the technicians train them. Charges for the use of the facility are based upon personnel time (including setup) and the cost of supplies. EHS Center support funds key personnel and defrays the costs of equipment maintenance. Charges to center members are 50% to 75% lower than to other academic users. Center members comprise 30-50% of all users; about 25 EHS Center members, from all four Research Cores, have used the Facility. Several examples were given of projects which highlight the importance of this Facility to the Center s interdisciplinary research. The Facility was recently expanded with new instruments and with overhauls of existing units. Future plans include: (1) upgrade of the Meridian ACAS system optics and computer; (2) replacement of the Coulter Profile II unit with an new instrument; and (3) expansion of current mini-courses and hands-on tutorials in methods of flow cytometry and image analysis.