CORE--NUCLEIC ACIDS AND PROTEINS

描述：该核心为中心调查人员提供访问最新情况的途径- 用于测定大分子结构的ART仪器，小型 分子结晶学、分子建模、瞬时动力学研究和 分子间相互作用。该设备包括最新的X光机 带有R-AXIS-IV图像板探测器和低温系统的衍射仪 Silicon Graphics工作站支持的低温数据采集 数据收集和数据处理以及适当的软件系统。一个 配备齐全的西门子P4衍射仪可用于小分子 结晶学。用于结构分析的仪器还包括600 MHZ核磁共振波谱仪(Brucker)，具有多种探头和SG1 用于数据采集的工作站。分子力学软件包 还可以进行动力学计算和3-D建模。包括在 用于结构测定的仪器也是UV环形的 二色性和傅里叶变换红外光谱仪。 两台IAsys仪器，一台手动，一台自动，可在 用于研究分子间相互作用的生物传感器设备。瞬变 动力学研究可以使用Dionex D-150温度跳跃进行 系统和两台停流仪表。所有必要的注射器和混合器 可用于接近1毫秒的死区时间。荧光发射， 圆二向色性和吸光度可以独立测量或 同时。可测量荧光偏振度和时序 可以进行研究。系统有温度控制和接口 以供计算机进行数据采集和分析。一种具有以下功能的荧光分光光度计 测量荧光强度、激发和发射光谱，以及 包括荧光各向异性。

Description: This Core provides access for Center investigators to state-of-the- art instrumentation for determination of macromolecular structure, small molecule crystallography, molecular modeling, study of transient kinetics and intermolecular interactions. The equipment includes an up-to-date X-ray diffractometer with an R-AXIS-IV image plate detector and cryogenic system for low temperature data collection supported by Silicon Graphics workstations for both data collection and data processing and appropriate software systems. A well outfitted Siemens P4 diffractometer is available for small molecule crystallography. Instrumentation for structural analysis also includes a 600 MHZ NMR spectrometer (Brucker) with a versatile collection of probes and SG1 workstation for data collection. Software packages for molecular mechanics and dynamics calculations and 3-D modeling are also available. Included in the instrumentation for structure determination are also a UV circular dichroism and Fourier transform IR spectrometers. Two IAsys instruments, one manual and one automated are available in the biosensor facility for study of intermolecular interactions. Transient kinetic studies can be carried out using a Dionex D-150 temperature jump system and two stopped-flow instruments. All necessary syringes and mixers are available to approach a dead time of 1 msec. Fluorescence emission, circular dichroism and absorbency can be measured independently or simultaneously. Fluorescence polarization can be measured and quence-flow studies can be carried out. Systems are temperature controlled and interfaced to computers for data acquisition and analysis. A spectrofluorometer capable of measuring fluorescence intensity excitation and emission spectra and fluorescence anisotrophy is included.