Mouse Model--Targeted Gene Knock-Out of Prostaglandin Sy

工作总结：一系列阐明单个环氧合酶（考克斯）异构体生理功能的项目正在考克斯基因敲除小鼠中进行。已经产生了考克斯-1和考克斯-2缺陷的小鼠。这些小鼠和仅携带一个考克斯基因拷贝的小鼠被用于确定单个考克斯同种型的生理作用，并阐明两种同种型单独或联合作用的方式。这两种亚型缺陷的小鼠在动脉导管未闭出生后不久死亡，但在其他方面表现正常。我们已经发现，考克斯-2是主要负责导管闭合的同种型，并且在导管的收缩的光滑肌中诱导考克斯-2，而不是考克斯-1

Summary of Work: A series of projects to elucidate the physiological functions of the individual cyclooxygenase (COX) isoforms are ongoing with the COX knockout mice. Mice deficient in both COX-1 and COX-2 have been produced. These mice, and mice carrying only a single copy of one of the COX genes, are being used to determine the physiological roles of the individual COX isoforms and to elucidate the ways in which the two isoforms act individually or in conjunction. Mice deficient in both isoforms die shortly after birth of patent ductus arteriosus, but otherwise appear developmentally normal. We have found that COX-2 is the isoform primarily responsible for the closure of the ductus and that COX-2, but not COX-1, is induced in the contracting smooth mucles of the ductus