IMPROVING SURFACE PLASMON RESONANCE TECHNOLOGY

描述：（改编自申请人摘要）本申请 代表了一种跨学科的努力，批判性地评估和改进 表面等离子体共振设计和分析的当前方法 (SPR)实验，因为它们被用来研究蛋白质：蛋白质相互作用。 SPR提供了一种强有力的技术来确定大分子的动力学 相互作用，实验设置往往意味着测量数据 偏离基于基础动力学模型预测的值。这些 偏差可能导致对实验数据的不正确分析。的 偏差可能来自多种来源，例如质量运输， 再结合效应目前建议在以下条件下进行动力学实验： 导致低信噪比的条件，这可能导致进一步的 数据分析的困难。据推测， 在信号处理文献中已经开发的技术可以 解决存在的一些问题。本申请的目的是：1） 研究参考细胞扣除和基线漂移对 测量的动力学常数，并开发克服 潜在的问题; 2）制定方法，以改善现有的 处理质量传输、再结合、低信噪比的技术 和快速关闭率; 3）开发进一步的方法来识别 给定蛋白质的校正相互作用模型：蛋白质相互作用;以及4） 开发一个软件环境，在其中这些研究可以有效地 贯彻

DESCRIPTION: (Adapted from the applicant's abstract) This application represents an interdisciplinary effort to critically evaluate and improve on current methodologies for the design and analysis of surface plasmon resonance (SPR) experiments as they are used to investigate protein:protein interactions. SPR offers a powerful technique to determine the kinetics of macromolecular interactions, the experimental set-up often implies that the measured data deviates from that predicted based on the underlying kinetic model. These deviations can lead to incorrect analysis of the experimental data. The deviations can arise from a multitude of sources, such as mass transport and rebinding effects. It is currently recommended to run kinetic experiments under conditions that result in a low signal to noise ratio which can lead to further difficulties in the data analysis. It is hypothesized that the application of techniques which have been developed in the signal processing literature can resolve some of the existing problems. The aims of this application are to: 1) to investigate the effects of reference cell subtraction and baseline drift on the measured kinetic constants and to develop methods that overcome the potential problems; 2) to develop methods to improve on the currently available techniques to deal with mass transport, rebinding, low signal to noise ratios and fast off rates; 3) to develop further methods for the identification of the correction interaction model for a given protein:protein interaction; and 4) to develop a software environment in which these studies can be efficiently carried out.