Post-transcriptional responses to cellular stresses by growth factor receptor genes

信使RNA的非翻译区中的调节元件允许对细胞应激和其他刺激的转录后应答。这种类型的调节允许细胞对压力做出反应，但也可能与不适当的基因表达和疾病有关。然而，更好地了解与疾病相关的基因的调控也使我们能够确定新的药物靶点，并设计改进的增殖性疾病的治疗方法。主要假设是，细胞应激反应中所需的基因将对通常伴随细胞应激的基因表达下调免疫。该项目将使用生物信息学和实验室工具的组合来研究EGFR/ErbB/HER生长因子受体家族如何响应细胞应激。这些生长因子受体的基因将被克隆，并用于报告基因测定和突变分析的组合，以确定调控元件。

Regulatory elements in the untranslated regions of messenger RNAs allow post-transcriptional responses to cellular stresses and other stimuli. This type of regulation allows cells to respond to stresses, but can also be linked with inappropriate gene expression and disease. However, a better understanding of the regulation of genes associated with disease also allows us to identify novel drug targets and to design improved therapies for proliferative disease. The principle hypothesis is that genes required in the response to cell stresses will be immune to the downregulation of gene expression that typically accompanies cell stresses. This project will use a combination of bioinformatic and laboratory tools to investigate how the EGFR/ErbB/HER family of growth factor receptors respond to cell stresses. The genes for these growth factor receptors will be cloned and used in a combination of reporter assays and mutational analyses to identify regulatory elements.