IDENTIFICATION OF PROTEIN CODING GENES IN HUMAN GENOMES

描述(改编自调查人员的摘要)：大规模的努力是 正在进行中，以完成整个人类基因组的排序。鉴定 通过计算工具对功能序列元素的分析已变得越来越多 很重要。研究人员的长期目标是使用数学和 在人类基因组中识别人类蛋白质编码基因的统计方法。 这需要定位它们的大致位置，这些位置由启动子和 多聚腺苷酸化信号；根据外显子描述它们的组织， 内含子和编码序列；并通过以下方法推断其结构/功能 研究它们的控制/调节元件及其编码的蛋白质。这个 调查人员为这次更新提出了以下三个具体目标 应用：(1)开发数据库和计算方法 人类和小鼠蛋白质编码基因第一外显子的鉴定。(2)至 开发一种基于进化的检测近端启动子的算法 研究几组特征良好的组织/发育特异体 脊椎动物的基因。(3)开发数据库和计算方法 人和人的选择性剪接外显子和转录本的鉴定 小鼠蛋白质编码基因。调查人员预测，方法和工具 这个项目的结果将帮助分子生物学家找到人类基因 并更有效和更准确地解释结构/功能。

DESCRIPTION (Adapted from the investigator's abstract): A large-scale effort is underway to complete the sequencing of the entire human genome. Identification of functional sequence elements by computational tools has become increasingly important. The investigators' long term goal is to use mathematical and statistical methods to identify human protein coding genes in the human genome. This entails locating their approximate positions bound by promoters and polyadenylation signals; delineating their organization in terms of exons, introns and coding sequences; and inferring their structure/function by examining their control/regulatory elements and their encoded proteins. The investigators propose the following three specific aims for this renewal application: (1) To develop database and computational methods for identification of first-exons in human and mouse protein-coding genes. (2) To develop an evolutionary based algorithm for detecting proximal-promoters by studying a few well-characterized sets of tissue/developmental specific vertebrate genes. (3) To develop database and computational methods for identification of alternatively spliced exons and transcripts in human and mouse protein coding genes. The investigators predict that methods and tools resulting from this project will help molecular biologists to find human genes and to interpret the structures/functions more efficiently and more accurately.