STABILITY OF TETANUS AND DIPHTHERIA TOXINS USING IMMUNOELECTROPHORESIS METHODS

毒素历来被认为是稳定的制剂； 然而，随着联合疫苗的制备和生产 这些疫苗产生的抗体或抗毒素，其特征和 随后毒素和抗毒素的稳定性变得更加相关。 Vero细胞试验中白喉毒素稳定性的检查 今年实施了一种改进的化验方法，其中包括冻干 抗毒素和特定的设定效力。使用这种方法，毒素稀释 在零下20摄氏度时非常稳定，但在 解冻和重新冻结。重组参照马的研究 抗白喉毒素(大鼠)已表明，该制剂不是 在2-8摄氏度下稳定6个月以上。这一发现 用于确保毒素和抗毒素制剂稳定性 用于细胞分析。

Toxins have historically been considered to be stable preparations; however, with the preparation of combination vaccines and the production of antibodies or antitoxins by these vaccines, the characterization and subsequent stability of toxins and antitoxins has become more relevant. Examination of diphtheria toxin stability in the Vero cell test was implemented this year using a modified assay that included freeze-dried antitoxins and specific set potencies. Using this method, toxin dilutions were found to be very stable at -20 degrees C, but lost potency after thawing and refreezing. Studies on the reconstituted Reference Horse Antitoxin (RAT) for Diphtheria have indicated that this preparation is not stable for more than 6 months at 2-8 degrees C. This finding has been utilized to insure that stability of the toxin and antitoxin preparations used in the cell assay.