Immune Response Regulation by Rhinoviruses

描述：（改编自研究者的摘要）：鼻病毒的3A和2B蛋白抑制宿主细胞的分泌器官。鼻病毒是哮喘患者呼吸系统疾病的主要原因，鼻病毒对哮喘的加重一直是许多临床研究的主题。这些研究揭示了研究中使用的病毒株与鼻病毒在哮喘中的作用得出的结论之间的相关性。我的研究将验证一个假设，即那些加剧哮喘的鼻病毒株也不能阻止蛋白质分泌，从而释放免疫信使和炎症。我将使用分子遗传学测试几种鼻病毒株的3A和2B蛋白的分泌抑制作用。然后，我将分离3A和/或2B的允许分泌的等位基因，并在亲本病毒的背景下使用报告蛋白进行分泌功能测试，以及在鼻病毒感染的细胞中测试免疫信使的细胞运输的功能分析。

DESCRIPTION: (Adapted from the Investigator's abstract): The 3A and 2B proteins from rhinovirus inhibit the secretory apparatus of the host cell. Rhinovirus is a major cause of respiratory problems in asthma patients, and the exacerbation of asthma by rhinovirus has been the subject of many clinical studies. Those studies reveal a correlation between the strain of virus used in the study and the conclusion drawn about the role of rhinovirus in asthma. My research will test the hypothesis that those rhinovirus strains which exacerbate asthma also fail to block protein secretion, allowing release of immune messengers and inflammation. I will use molecular genetics to test the 3A and 2B proteins from several rhinovirus strains for secretory inhibition. I will then isolate secretion-permissive alleles of 3A and/or 2B, and test them in the context of the parent viruses using reporter proteins for secretory function as well as functional assays testing cellular transport of immune messengers in rhinovirus-infected cells.