Innovative Technology for Gene Expression Studies

描述(由申请人提供)： Alderon Biosciences建议开发一种新的电化学方法来处理 检测特定的核酸序列而不需要酶、荧光或 并展示了将该方法应用于 用于基因表达研究的电化学板(E板)格式 癌症。E板，传统微板的电气模拟，以及 Reader，将被开发为识别、量化和研究mRNAs和 对癌症研究特别感兴趣的cdna文库。寡核苷酸 含有电活性碱基的探针将被用来捕获目标 通过在E盘的单独可寻址元件上进行杂交来获得序列。 探针-靶标杂交在缓冲液存在的情况下产生信号 包含一名调解人。该信号可被快速读出并用于 评估差异基因表达模式。预期的结果是 创新的电化学板和读取器，为研究人员提供 一种快速、简便、经济的检测小样本特异性mRNA的方法 或cdna序列。拟议方法的另一个显著特点是 将在人类肿瘤抑制基因靶点的研究中得到证实 从E板井中恢复捕获目标的能力 随后进行克隆、测序或质谱分析。

DESCRIPTION (provided by applicant): Alderon Biosciences proposes to develop a new electrochemical method for detection of specific nucleic acid sequences without enzymatic, fluorescent or radioactive labels and to show the feasibility of applying this method in an electrochemical plate (E-Plate) format to studies of gene expression in cancer. The E-Plate, an electrical analog of a traditional microplate and reader, will be developed as a tool to identify, quantify and study mRNAs and cDNA libraries of specific interest for cancer studies. Oligonucleotide probes containing electroactive bases will be used to capture the target sequences by hybridization on separately addressable elements of the E-Plate. Probe-target hybridization generates a signal in the presence of a buffer containing a mediator. The signal can be read out rapidly and be used to evaluate differential gene expression patterns. The expected result is an innovative electrochemical plate and reader that provides researchers with a rapid, facile and affordable method to probe small samples for specific mRNA or cDNA sequences. Another distinctive feature of the proposed approach that will be demonstrated in studies with human tumor suppressor mRNA targets is the ability to recover captured targets from the wells of the E-Plate for subsequent cloning, sequencing, or mass spectrometric analysis.