Biomarker Chip for Osteoarthritis

描述（由申请人提供）：骨关节炎（OA）是老年人中最常见的肌肉骨骼疾病，以慢性疼痛和终身残疾为特征。目前诊断骨性关节炎的“金标准”是放射学，但放射学变化发生在导致疼痛和残疾的骨组织变化已经发生之后，相对较晚。使用OA生物标志物进行早期检测的诊断测试将有助于早期诊断和治疗，改善症状并可能减轻残疾。该项目的总体目标是生产一种用于检测血清中OA生物标志物蛋白质的微阵列，包括细胞因子和软骨和骨分解代谢产物。为了达到足够的灵敏度，该微阵列将利用一种新颖的专有技术，滚动圈放大，用于信号放大。启用rca的微阵列将提供多路复用（同时测量约100种分析物），减少样品体积（仅15 ul/assay），高通量和卓越灵敏度（约10 pg/mI）的优点。最初的研究将使用OA患者的血清和我们现有的细胞因子芯片进行适当的对照。我们还将确定其他试剂作为OA潜在有用的生物标志物，目的是设计OA芯片。

DESCRIPTION (provided by applicant): Osteoarthritis (OA) is the most prevalent musculo-skeletal disease in the older adult population, characterized by chronic pain and life long disability. The current 'gold standard' for diagnosis of OA is radiology, but radiological changes occur relatively late in the disease after the bone tissue changes responsible for the pain and disability have already taken place. A diagnostic test that uses biomarkers of OA for early detection would be useful in early diagnosis and treatment, ameliorating symptoms and potentially lessening disability. The general goal of this project is to produce a microarray for detecting proteins in serum that are biomarkers of OA including cytokines and products of cartilage & bone catabolism In order to achieve sufficient sensitivity, this microarray will utilize a novel proprietary technology, rolling circle amplification, for signal amplification. The RCA-enabled microarray will offer the benefits of multiplexing (measuring approximately 100 analytes simultaneously), reduced sample volume (only 15 ul/assay), high-throughput, and exceptional sensitivity (ca. 10 pg/mI). Initial studies will be done with serum from patients with OA and appropriate controls using our current cytokine chip. We also will identify other reagents as potentially useful biomarkers for OA with the aim of designing an OA chip.