CORE--MORPHOLOGY

形态学/形态测量学核心实验室旨在促进 组织学、免疫组织化学、原位杂交、 以及拟议项目所需的形态测量研究。这一核心将 提供服务、咨询、技术援助和/或培训 PPG调查者传导细胞的形态和成像技术 组织学和超微结构的生物学和形态研究 水平。这个实验室拥有全系列的光和电子。 显微镜、辅助仪器和制备资源以及 用于通勤人员辅助图像、形态测量和 密度分析。实验室的工作人员都是合格的， 经验丰富的技术人员将加工组织用于照明和 包括免疫组织化学和其他组织化学的透射电子显微镜 染色和原位杂交分析。核心董事将 执行项目II所需的大部分形态测量分析 这一核心的两个关键顾问包括达拉斯·海德博士，他 提供肺和大卫的体视学和形态测量方面的专业知识 米尔霍恩博士，他提供原位杂交方面的专业知识。所有的 主要项目和大部分试点和可行性项目将 很大程度上依赖于这个单位。这一核心的多样性和成本效益 直接归因于与其他研究单位的共同支持 使用这一设施，包括儿科肺SCOR、CF SCOR和 环境医学和肺部生物学中心。

The Morphology/Morphometry Core laboratory is designed to facilitate performance of morphologic, immunohistochemical, in situ hybridization, and morphometric studies needed for the proposed projects. This Core will provide service, consultation, technical assistance, and or training morphologic and imaging techniques for PPG investigators conducting cell biological and morphologic studies at the histologic and ultrastructural level. This laboratory maintains a full range of light and electron microscopes, ancillary apparatus and preparative resources as well as hardware and software for commuter-assisted image, morphometric, and densitometric analyses. The laboratory is staffed by well-qualified, experienced technicians who will process tissues for light and transmission electron microscopy including immuno- and other histochemical staining and in situ hybridization analyses. The Core director will perform the majority of the morphometric analyses needed by projects II and III. Two key consultants for this Core include Dallas Hyde, Ph.D. who provides expertise in stereology and morphometry of the lung and David Millhorn, Ph.D. who provides expertise in in situ hybridization. All of the major projects and most of the Pilot and Feasibility projects will rely heavily on this unit. This Core's diversity and cost-effectiveness are directly attributable to shared support with other research units using this facility, including a Pediatric Pulmonary SCOR, CF SCOR, and the Center for Environmental Medicine and Lung Biology.