CORE--ISOLATED MYOCYTE AND WHOLE HEART PHYSIOLOGY

该核心的两个总体目标之一是为SCOR的项目负责人提供设施，专业知识和技术支持，以分离心脏细胞并确定分离心肌细胞的收缩功能和钙稳态。这些实验将主要在基因工程小鼠的心脏细胞上进行，并与野生型对照进行比较，尽管也将使用大鼠心脏细胞。将提供一些心脏细胞制备物用于随后的生化分析（例如，酶测定、Northern和Western印迹），这些分析将由各自SCOR项目的研究者进行。第二个主要的超声心动图，并在以后的几年中，测量体内血液动力学参数的小鼠从各种小鼠线。因此，核心B将支持SCOR项目负责人在单细胞水平以及整个动物背景下评估其各自小鼠模型的表型。具体目标是：1。从新生和成年小鼠和大鼠的心房和心室组织中分离并启动心肌细胞的原代培养。2.测量来自野生型和基因工程小鼠的分离的心肌细胞中的收缩功能和细胞内[Ca2+]i。3.在野生型和基因工程小鼠中进行M型、二维和多普勒超声心动图研究。4.测量野生型和基因工程小鼠的血液动力学参数（如血压、主动脉流量、心输出量、压力容积环）。5.对扩张型心肌病家系和先证者的心功能进行临床无创性评价。

One of the two overall objectives of this Core is to provide project leaders in the SCOR with facilities, expertise and technical support for isolating heart cells and determining contractile function and calcium homeostasis in isolated cardiomyocytes. These experiments will be done mainly on heart cells from genetically engineered mice and compared to wild type controls, although rat heart cells will also be used. Some heart cell preparations will be provide for subsequent biochemical analyses (such as, for example, enzyme assays, Norther and Western blots) which will be performed by investigators of the respective SCOR projects. The second main echocardiography and, in later years, measuring in vivo hemodynamic parameters in mice from the various mouse lines. Core B will therefore support SCOR project leaders in assessing the phenotype of their respective mouse models, both on a single cell level as well as in the whole animal context. The Specific Aims are: 1. Isolate and initiate primary cultures of cardiac myocytes from atrial and ventricular tissue of neonatal and adult mice and rats. 2. Measure contractile function and intracellular [Ca2+]i in isolated cardiomyocytes from wild type and genetically engineered mice. 3. Perform M-mode, two dimensional and Doppler echocardiography studies in wild type and genetically engineered mice. 4. Measure hemodynamic parameters (such as blood pressure, aortic flow, cardiac output, pressure volume loops) in wild type and genetically engineered mice. 5. Provide clinical non-invasive evaluation of cardiac function in study families and probands with dilated cardiomyopathy.