Using genomic and postgenomic tools to identify the geomicrobiological basis of arsenic mobilisation in aquifers

作为与其他主要砷团体开展的一项重大国际合作方案的一部分，将采用最先进的取样程序，从砷浓度高的含水层中获取沉积物岩心和地下水，并将其保存起来，以便在曼彻斯特进行详细分析。将进行分子生态学研究，包括使用16 S rRNA或功能基因扩增子的照明测序对微生物群落进行系统发育和功能分析。学生还将开发和应用宏基因组学，转录组学和蛋白质组学方法来分析这些样品，以帮助阐明砷动员的原位机制。将对这些样品进行平行的矿物学和地球化学分析，并在精心设计的微观研究中支持这项工作，并帮助建立一个含水层沉积物中砷动员的综合模型。该学生将在地质微生物学家，地球化学家和矿物学家团队的监督下工作，并将成为曼彻斯特地下微生物学和砷地球化学工作的一组学术和技术人员，博士后和博士生的一部分。他们将设在威廉姆森分子环境科学研究中心的最先进的实验室，并将受益于生命科学学院的后基因组科学的优良基础设施。学生将产生一系列高影响力的出版物，并将在分子环境科学的职业生涯做好准备。参考文献（1）伊斯兰教，F.S.，Gault，A.G.，Boothman，C.，波利亚地方检察官Charnock，J.M.，Chatterjee，D.和劳埃德，J.R.（2004）金属还原细菌在孟加拉三角洲沉积物中砷释放的作用。第68-71节：一个人的世界(2)Lear，G.，宋，B.，Gault，A.G.，Polya，D.A.和J.R. Lloyd（2007年），柬埔寨沉积物中的砷酸盐还原菌的分子分析，经醋酸盐修正后。应用和环境微生物学731041-104。(3)汉德利，K.M.，McBeth，J.M.，Charnock，J.M.，沃恩，D. J.，Wincott，P.L.，Polya，D.A.和Lloyd，J.R.（2013）铁氧化还原转化对富砷含铁热液沉积物中砷固相缔合的影响。Geochimica et Cosmochimica Acta 102 124-142

As part of a major collaborative international programme with other leading arsenic groups, sediment cores and groundwaters will be obtained from aquifers with high arsenic concentrations, using state of the art sampling procedures and preserved for detailed analyses in Manchester. Molecular ecology studies will be conducted, including phylogenetic and functional profiling of microbial communities using illumine sequencing of 16S rRNA or functional gene amplicons. The student will also develop and apply metagenomic, transcriptomic and proteomic approaches for analyses of these samples, to help elucidate the in situ mechanism of arsenic mobilization. Parallel mineralogical and geochemical analyses will be conducted on these samples, and in carefully-designed microcosm studies to support this work, and help build a comprehensive model for the mobilisation of arsenic in aquifer sediments. The student will work under the supervision of a team of geomicrobiologists, geochemists and mineralogists and will be part of a group of academic and technical staff, postdocs and PhD students working on subsurface microbiology and arsenic biogeochemistry at Manchester. They will be based in state of the art laboratories in the Williamson Research Centre for Molecular Environmental Science, and will also benefit from the excellent infrastructure for post-genomic science in the Faculty of Life Sciences. The student will generate a series of high impact publications and will be well prepared for a career in molecular environmental science.References (1) Islam, F.S., Gault, A.G., Boothman, C., Polya, D.A., Charnock, J.M., Chatterjee, D. and Lloyd, J.R. (2004) Role of metal-reducing bacteria in arsenic release from Bengal Delta sediments. Nature 430 68-71. (2) Lear, G., Song, B., Gault, A.G., Polya, D.A. and J.R. Lloyd (2007) Molecular Analysis of Arsenate-Reducing Bacteria within Cambodian Sediments Following Amendment with Acetate. Applied and Environmental Microbiology 731041-104. (3) Handley, K.M., McBeth, J.M., Charnock, J.M., Vaughan, D.J., Wincott, P.L., Polya, D.A. and Lloyd, J.R. (2013) Effect of iron redox transformations on arsenic solid-phase associations in an asenic-rich, ferruginous hydrothermal sediment. Geochimica et Cosmochimica Acta 102 124-142