Core--Animal

描述（由申请人提供） 动物核心设施将作为研究中心PPG的组成部分。 它由肺部和血液系统疾病的啮齿动物模型组成， 将被所有项目使用。该核心将提供体内模型， 研究者在启动前建立必要的临床前研究 临床试验动物核心将提供的一些服务是 与获得用于气道和血友病基因治疗的新动物模型有关 （项目3和4）通过转基因和敲除/敲入技术。 这些技术是一项既定的服务，将提供给调查人员， 缺乏创造这些宝贵资源的设施或专业知识。 动物的繁殖、饲养和分布是本组织的主要目标。 核心设施，并将用于项目1，2，3和4。除了 动物的日常饲养和维护，高度 动物中心的训练有素的人员提供了额外的好处， 研究计划。动物模型中心提供高质量的 以可控的成本提供服务。训练有素的工作人员直接与 促进研究人员的合作和资源共享。也许 最重要的是，该基金在许多层面上提供援助和指导 (i.e.创造转基因小鼠，从构建DNA结构， 动物的表型表征）。因此，我们可以防止 这些建议概念简单，但技术要求高， 成为他们成功的严重障碍。鼠标设备具有出色的 成功创建、维护和繁殖动物模型的记录， 这些资源将为实现可持续发展方案的总体目标增加价值。

DESCRIPTION (provided by applicant) The Animal Core Facility will serve as an integral component of the UNC PPG. It is composed of rodent models for Pulmonary and Hematologic disorders and will be utilized by all projects. This core will provide in vivo models to the investigator for establishing preclinical studies necessary before initiating clinical trials. Some of the services the animal core will provide are related to deriving new animal models for airway and hemophilia gene therapy (Projects 3 and 4) through transgenic and knock out/knock in technology. These techniques are an established service to be offered to investigators who lack the facilities or expertise to create these invaluable resources. Breeding, maintaining, and distribution of animals is a major objective of the core facility and will be used by Projects 1, 2, 3, and 4. Apart from the routine breeding and maintaining of animals, the availability of the highly trained personnel of the Animal Core provides additional benefits to this research program. The UNC Animal Models Core Facility provides top-quality services at a manageable cost. Highly trained staff is directly engaged with researchers, and collaborations and resource sharing are facilitated. Perhaps most importantly, the facility provides assistance and guidance at many levels (i.e. creating genetically modified mice, from building, a DNA construct to phenotypic characterization of the animals). Thus, we can prevent a conceptually simple but technically demanding aspect of these proposals from becoming a serious obstacle to their success. The mouse facility has excellent records of success in creating, maintaining, and breeding animal models and these resources will be an added value to the overall goal of the UNC PPG.